tetano
Editor, Senior Moderator
Sci China Life Sci
. 2021 Apr 28.
doi: 10.1007/s11427-020-1921-5. Online ahead of print.
Longitudinal virological changes and underlying pathogenesis in hospitalized COVID-19 patients in Guangzhou, China
Zhengtu Li[SUP] #[/SUP][SUP] 1 [/SUP], Yinhu Li[SUP] #[/SUP][SUP] 2 [/SUP], Ruilin Sun[SUP] #[/SUP][SUP] 3 [/SUP], Shaoqiang Li[SUP] #[/SUP][SUP] 1 [/SUP], Lingdan Chen[SUP] #[/SUP][SUP] 1 [/SUP], Yangqing Zhan[SUP] #[/SUP][SUP] 1 [/SUP], Mingzhou Xie[SUP] 4 [/SUP], Jiasheng Yang[SUP] 3 [/SUP], Yanqun Wang[SUP] 1 [/SUP], Airu Zhu[SUP] 1 [/SUP], Guoping Gu[SUP] 1 [/SUP], Le Yu[SUP] 4 [/SUP], Shuaicheng Li[SUP] 2 [/SUP], Tingting Liu[SUP] 4 [/SUP], Zhaoming Chen[SUP] 1 [/SUP], Wenhua Jian[SUP] 1 [/SUP], Qian Jiang[SUP] 1 [/SUP], Xiaofen Su[SUP] 1 [/SUP], Weili Gu[SUP] 1 [/SUP], Liyan Chen[SUP] 1 [/SUP], Jing Cheng[SUP] 1 [/SUP], Jincun Zhao[SUP] 1 [/SUP], Wenju Lu[SUP] 1 [/SUP], Jinping Zheng[SUP] 1 [/SUP], Shiyue Li[SUP] 1 [/SUP], Nanshan Zhong[SUP] 1 [/SUP], Feng Ye[SUP] 5 [/SUP]
Affiliations
Abstract
Prolonged viral RNA shedding and recurrence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in coronavirus disease 2019 (COVID-19) patients have been reported. However, the clinical outcome and pathogenesis remain unclear. In this study, we recruited 43 laboratory-confirmed COVID-19 patients. We found that prolonged viral RNA shedding or recurrence mainly occurred in severe/critical patients (P<0.05). The average viral shedding time in severe/critical patients was more than 50 days, and up to 100 days in some patients, after symptom onset. However, chest computed tomography gradually improved and complete absorption occurred when SARS-CoV-2 RT-PCR was still positive, but specific antibodies appeared. Furthermore, the viral shedding time significantly decreased when the A1,430G or C12,473T mutation occurred (P<0.01 and FDR<0.01) and increased when G227A occurred (P<0.05 and FDR<0.05). High IL1R1, IL1R2, and TNFRSF21 expression in the host positively correlated with viral shedding time (P<0.05 and false discovery rate <0.05). Prolonged viral RNA shedding often occurs but may not increase disease damage. Prolonged viral RNA shedding is associated with viral mutations and host factors.
Keywords: SRAS-CoV-2; host gene expression; longitudinal virological changes; viral genome
. 2021 Apr 28.
doi: 10.1007/s11427-020-1921-5. Online ahead of print.
Longitudinal virological changes and underlying pathogenesis in hospitalized COVID-19 patients in Guangzhou, China
Zhengtu Li[SUP] #[/SUP][SUP] 1 [/SUP], Yinhu Li[SUP] #[/SUP][SUP] 2 [/SUP], Ruilin Sun[SUP] #[/SUP][SUP] 3 [/SUP], Shaoqiang Li[SUP] #[/SUP][SUP] 1 [/SUP], Lingdan Chen[SUP] #[/SUP][SUP] 1 [/SUP], Yangqing Zhan[SUP] #[/SUP][SUP] 1 [/SUP], Mingzhou Xie[SUP] 4 [/SUP], Jiasheng Yang[SUP] 3 [/SUP], Yanqun Wang[SUP] 1 [/SUP], Airu Zhu[SUP] 1 [/SUP], Guoping Gu[SUP] 1 [/SUP], Le Yu[SUP] 4 [/SUP], Shuaicheng Li[SUP] 2 [/SUP], Tingting Liu[SUP] 4 [/SUP], Zhaoming Chen[SUP] 1 [/SUP], Wenhua Jian[SUP] 1 [/SUP], Qian Jiang[SUP] 1 [/SUP], Xiaofen Su[SUP] 1 [/SUP], Weili Gu[SUP] 1 [/SUP], Liyan Chen[SUP] 1 [/SUP], Jing Cheng[SUP] 1 [/SUP], Jincun Zhao[SUP] 1 [/SUP], Wenju Lu[SUP] 1 [/SUP], Jinping Zheng[SUP] 1 [/SUP], Shiyue Li[SUP] 1 [/SUP], Nanshan Zhong[SUP] 1 [/SUP], Feng Ye[SUP] 5 [/SUP]
Affiliations
- PMID: 33945070
- DOI: 10.1007/s11427-020-1921-5
Abstract
Prolonged viral RNA shedding and recurrence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in coronavirus disease 2019 (COVID-19) patients have been reported. However, the clinical outcome and pathogenesis remain unclear. In this study, we recruited 43 laboratory-confirmed COVID-19 patients. We found that prolonged viral RNA shedding or recurrence mainly occurred in severe/critical patients (P<0.05). The average viral shedding time in severe/critical patients was more than 50 days, and up to 100 days in some patients, after symptom onset. However, chest computed tomography gradually improved and complete absorption occurred when SARS-CoV-2 RT-PCR was still positive, but specific antibodies appeared. Furthermore, the viral shedding time significantly decreased when the A1,430G or C12,473T mutation occurred (P<0.01 and FDR<0.01) and increased when G227A occurred (P<0.05 and FDR<0.05). High IL1R1, IL1R2, and TNFRSF21 expression in the host positively correlated with viral shedding time (P<0.05 and false discovery rate <0.05). Prolonged viral RNA shedding often occurs but may not increase disease damage. Prolonged viral RNA shedding is associated with viral mutations and host factors.
Keywords: SRAS-CoV-2; host gene expression; longitudinal virological changes; viral genome