tetano
Editor, Senior Moderator
Sci Adv
. 2025 Jul 4;11(27):eadu3739.
doi: 10.1126/sciadv.adu3739. Epub 2025 Jul 4. Mitochondrial dysfunction enhances influenza pathogenesis by up-regulating de novo sialic acid biosynthesis
Amanda L Fuchs[SUP] 1 [/SUP], Bharati Singh[SUP] 1 [/SUP], Jillian W Jetmore[SUP] 1 [/SUP], Emily B Warren[SUP] 1 [/SUP], Payal Banerjee[SUP] 1 [/SUP], Jose L Marin Franco[SUP] 1 [/SUP], Michael A Eckhaus[SUP] 2 [/SUP], Tatiana N Tarasenko[SUP] 1 [/SUP], Madeleine R Assaad[SUP] 1 [/SUP], Ivan Kosik[SUP] 3 [/SUP], Jonathan Yewdell[SUP] 3 [/SUP], Peter J McGuire[SUP] 1 [/SUP]
Affiliations
Mitochondrial dysfunction can trigger metabolic adaptations that resemble those induced by influenza A virus (IAV) infection. Here, we show that oxidative phosphorylation (OXPHOS) impairment, modeled by Ndufs4 deficiency, reprograms lung epithelial metabolism to promote IAV pathogenesis. In both Ndufs4 knockout (KO) mice and lung epithelial cells, OXPHOS deficiency increased glycolytic flux, diverting carbons into hexosamine and de novo sialic acid (SIA) biosynthesis pathways. This led to elevated sialylation and enhanced viral attachment. In Ndufs4 KO models, adenosine monophosphate-activated protein kinase signaling was insufficient to blunt this increased metabolic flux. IAV infection further exacerbated this metabolic vulnerability, amplifying SIA and viral burden. Pharmacologic rerouting of glucose carbons with dichloroacetate reduced sialylation, viral replication, and inflammatory responses in Ndufs4 KO models. These findings reveal that mitochondrial dysfunction enhances IAV susceptibility by disrupting energy sensing and fueling viral receptor biosynthesis, highlighting the importance of epithelial metabolism in viral pathogenesis and suggesting metabolic modulation as a potential therapeutic.
. 2025 Jul 4;11(27):eadu3739.
doi: 10.1126/sciadv.adu3739. Epub 2025 Jul 4. Mitochondrial dysfunction enhances influenza pathogenesis by up-regulating de novo sialic acid biosynthesis
Amanda L Fuchs[SUP] 1 [/SUP], Bharati Singh[SUP] 1 [/SUP], Jillian W Jetmore[SUP] 1 [/SUP], Emily B Warren[SUP] 1 [/SUP], Payal Banerjee[SUP] 1 [/SUP], Jose L Marin Franco[SUP] 1 [/SUP], Michael A Eckhaus[SUP] 2 [/SUP], Tatiana N Tarasenko[SUP] 1 [/SUP], Madeleine R Assaad[SUP] 1 [/SUP], Ivan Kosik[SUP] 3 [/SUP], Jonathan Yewdell[SUP] 3 [/SUP], Peter J McGuire[SUP] 1 [/SUP]
Affiliations
- PMID: 40614187
- DOI: 10.1126/sciadv.adu3739
Mitochondrial dysfunction can trigger metabolic adaptations that resemble those induced by influenza A virus (IAV) infection. Here, we show that oxidative phosphorylation (OXPHOS) impairment, modeled by Ndufs4 deficiency, reprograms lung epithelial metabolism to promote IAV pathogenesis. In both Ndufs4 knockout (KO) mice and lung epithelial cells, OXPHOS deficiency increased glycolytic flux, diverting carbons into hexosamine and de novo sialic acid (SIA) biosynthesis pathways. This led to elevated sialylation and enhanced viral attachment. In Ndufs4 KO models, adenosine monophosphate-activated protein kinase signaling was insufficient to blunt this increased metabolic flux. IAV infection further exacerbated this metabolic vulnerability, amplifying SIA and viral burden. Pharmacologic rerouting of glucose carbons with dichloroacetate reduced sialylation, viral replication, and inflammatory responses in Ndufs4 KO models. These findings reveal that mitochondrial dysfunction enhances IAV susceptibility by disrupting energy sensing and fueling viral receptor biosynthesis, highlighting the importance of epithelial metabolism in viral pathogenesis and suggesting metabolic modulation as a potential therapeutic.