tetano
Editor, Senior Moderator
Sci Adv
. 2022 Nov 11;8(45):eabp9961.
doi: 10.1126/sciadv.abp9961. Epub 2022 Nov 11.
High-temporal resolution profiling reveals distinct immune trajectories following the first and second doses of COVID-19 mRNA vaccines
Darawan Rinchai[SUP] 1 2 [/SUP], Sara Deola[SUP] 1 [/SUP], Gabriele Zoppoli[SUP] 3 4 [/SUP], Basirudeen Syed Ahamed Kabeer[SUP] 1 [/SUP], Sara Taleb[SUP] 5 [/SUP], Igor Pavlovski[SUP] 1 [/SUP], Selma Maacha[SUP] 1 [/SUP], Giusy Gentilcore[SUP] 1 [/SUP], Mohammed Toufiq[SUP] 1 [/SUP], Lisa Mathew[SUP] 1 [/SUP], Li Liu[SUP] 1 [/SUP], Fazulur Rehaman Vempalli[SUP] 1 [/SUP], Ghada Mubarak[SUP] 1 [/SUP], Stephan Lorenz[SUP] 1 [/SUP], Irene Sivieri[SUP] 4 6 7 [/SUP], Gabriella Cirmena[SUP] 3 [/SUP], Chiara Dentone[SUP] 3 [/SUP], Paola Cuccarolo[SUP] 4 [/SUP], Daniele Roberto Giacobbe[SUP] 3 6 [/SUP], Federico Baldi[SUP] 6 [/SUP], Alberto Garbarino[SUP] 4 [/SUP], Benedetta Cigolini[SUP] 4 [/SUP], Paolo Cremonesi[SUP] 8 [/SUP], Michele Bedognetti[SUP] 9 [/SUP], Alberto Ballestrero[SUP] 3 6 [/SUP], Matteo Bassetti[SUP] 3 6 [/SUP], Boris P Hejblum[SUP] 10 [/SUP], Tracy Augustine[SUP] 1 [/SUP], Nicholas Van Panhuys[SUP] 1 [/SUP], Rodolphe Thiebaut[SUP] 10 [/SUP], Ricardo Branco[SUP] 1 [/SUP], Tracey Chew[SUP] 11 [/SUP], Maryam Shojaei[SUP] 12 13 14 [/SUP], Kirsty Short[SUP] 15 16 [/SUP], Carl G Feng[SUP] 17 18 [/SUP], PREDICT-19 Consortium; Susu M Zughaier[SUP] 19 [/SUP], Andrea De Maria[SUP] 3 6 [/SUP], Benjamin Tang[SUP] 13 [/SUP], Ali Ait Hssain[SUP] 20 21 [/SUP], Davide Bedognetti[SUP] 1 4 [/SUP], Jean-Charles Grivel[SUP] 1 [/SUP], Damien Chaussabel[SUP] 1 22 [/SUP]
Affiliations
Abstract
Knowledge of the mechanisms underpinning the development of protective immunity conferred by mRNA vaccines is fragmentary. Here, we investigated responses to coronavirus disease 2019 (COVID-19) mRNA vaccination via high-temporal resolution blood transcriptome profiling. The first vaccine dose elicited modest interferon and adaptive immune responses, which peaked on days 2 and 5, respectively. The second vaccine dose, in contrast, elicited sharp day 1 interferon, inflammation, and erythroid cell responses, followed by a day 5 plasmablast response. Both post-first and post-second dose interferon signatures were associated with the subsequent development of antibody responses. Yet, we observed distinct interferon response patterns after each of the doses that may reflect quantitative or qualitative differences in interferon induction. Distinct interferon response phenotypes were also observed in patients with COVID-19 and were associated with severity and differences in duration of intensive care. Together, this study also highlights the benefits of adopting high-frequency sampling protocols in profiling vaccine-elicited immune responses.
. 2022 Nov 11;8(45):eabp9961.
doi: 10.1126/sciadv.abp9961. Epub 2022 Nov 11.
High-temporal resolution profiling reveals distinct immune trajectories following the first and second doses of COVID-19 mRNA vaccines
Darawan Rinchai[SUP] 1 2 [/SUP], Sara Deola[SUP] 1 [/SUP], Gabriele Zoppoli[SUP] 3 4 [/SUP], Basirudeen Syed Ahamed Kabeer[SUP] 1 [/SUP], Sara Taleb[SUP] 5 [/SUP], Igor Pavlovski[SUP] 1 [/SUP], Selma Maacha[SUP] 1 [/SUP], Giusy Gentilcore[SUP] 1 [/SUP], Mohammed Toufiq[SUP] 1 [/SUP], Lisa Mathew[SUP] 1 [/SUP], Li Liu[SUP] 1 [/SUP], Fazulur Rehaman Vempalli[SUP] 1 [/SUP], Ghada Mubarak[SUP] 1 [/SUP], Stephan Lorenz[SUP] 1 [/SUP], Irene Sivieri[SUP] 4 6 7 [/SUP], Gabriella Cirmena[SUP] 3 [/SUP], Chiara Dentone[SUP] 3 [/SUP], Paola Cuccarolo[SUP] 4 [/SUP], Daniele Roberto Giacobbe[SUP] 3 6 [/SUP], Federico Baldi[SUP] 6 [/SUP], Alberto Garbarino[SUP] 4 [/SUP], Benedetta Cigolini[SUP] 4 [/SUP], Paolo Cremonesi[SUP] 8 [/SUP], Michele Bedognetti[SUP] 9 [/SUP], Alberto Ballestrero[SUP] 3 6 [/SUP], Matteo Bassetti[SUP] 3 6 [/SUP], Boris P Hejblum[SUP] 10 [/SUP], Tracy Augustine[SUP] 1 [/SUP], Nicholas Van Panhuys[SUP] 1 [/SUP], Rodolphe Thiebaut[SUP] 10 [/SUP], Ricardo Branco[SUP] 1 [/SUP], Tracey Chew[SUP] 11 [/SUP], Maryam Shojaei[SUP] 12 13 14 [/SUP], Kirsty Short[SUP] 15 16 [/SUP], Carl G Feng[SUP] 17 18 [/SUP], PREDICT-19 Consortium; Susu M Zughaier[SUP] 19 [/SUP], Andrea De Maria[SUP] 3 6 [/SUP], Benjamin Tang[SUP] 13 [/SUP], Ali Ait Hssain[SUP] 20 21 [/SUP], Davide Bedognetti[SUP] 1 4 [/SUP], Jean-Charles Grivel[SUP] 1 [/SUP], Damien Chaussabel[SUP] 1 22 [/SUP]
Affiliations
- PMID: 36367935
- DOI: 10.1126/sciadv.abp9961
Abstract
Knowledge of the mechanisms underpinning the development of protective immunity conferred by mRNA vaccines is fragmentary. Here, we investigated responses to coronavirus disease 2019 (COVID-19) mRNA vaccination via high-temporal resolution blood transcriptome profiling. The first vaccine dose elicited modest interferon and adaptive immune responses, which peaked on days 2 and 5, respectively. The second vaccine dose, in contrast, elicited sharp day 1 interferon, inflammation, and erythroid cell responses, followed by a day 5 plasmablast response. Both post-first and post-second dose interferon signatures were associated with the subsequent development of antibody responses. Yet, we observed distinct interferon response patterns after each of the doses that may reflect quantitative or qualitative differences in interferon induction. Distinct interferon response phenotypes were also observed in patients with COVID-19 and were associated with severity and differences in duration of intensive care. Together, this study also highlights the benefits of adopting high-frequency sampling protocols in profiling vaccine-elicited immune responses.