Mary Wilson
Well-known member
Posted October 10, 2023.
doi: https://doi.org/10.1101/2023.10.09.557914
Kamini Jakhar, Sudipta Sonar, Gagandeep Singh, Tania Sarkar, Mahima Tiwari, Jaskaran Kaur, Deepak Kumar Rathore, Banwari Lal, Sandeep Kumar, Puneet Srivastav, Satendra Kumar, Vikas Phagna, Lokesh Kumar, Vishal Gupta, Pallavi Kshetrapal, Savita Singh, Nitya Wadhwa, Ramachandran Thiruvengadam, Sreevatsan Raghavan, Mudita Gosain, Tripti Shrivastava, Sankar Bhattacharyya, Jayanta Bhattacharya, Shailendra Asthana, Shailendra Mani
Abstract
Dengue disease is highly prevalent in tropical and subtropical regions worldwide. However, its pathogenesis is still incompletely understood, particularly in comparison to other endemic viruses. Antibody-dependent enhancement (ADE) is a well-known phenomenon for dengue viruses. Given the recent surge in dengue cases and potential cross-reactivity with SARS-CoV-2 antibodies, this study explores the impact of anti-SARS-CoV-2 antibodies on DENV-2 infection. The study assessed the cross-reactivity of SARS-CoV-2 antibodies with the DENV-2 Virus. Human convalescent plasma samples collected during different waves of COVID-19 and monoclonal and polyclonal antibodies raised against SARS-CoV-2 were examined for their potential to cause ADE of DENV-2 infection using cell-based assays. The study found that anti-SARS-CoV-2 antibodies acquired from natural infection in humans or through experimental immunization in animals were cross-reactive with DENV-2 and had the potential to enhance DENV-2 infection in K562 and U937 cells. In-silico and in-vitro studies indicated a strong interaction between SARS-CoV-2 antibodies and DENV-2 E-protein, providing a molecular basis for these findings. This study is the first to demonstrate that anti-SARS-CoV-2 antibodies can cross-react with DENV-2 and can enhance its infection through ADE. These findings have implications for SARS-CoV-2 vaccine development and deployment strategies in regions where dengue is endemic.
https://www.biorxiv.org/content/10.1101/2023.10.09.557914v1.full.pdf
doi: https://doi.org/10.1101/2023.10.09.557914
Kamini Jakhar, Sudipta Sonar, Gagandeep Singh, Tania Sarkar, Mahima Tiwari, Jaskaran Kaur, Deepak Kumar Rathore, Banwari Lal, Sandeep Kumar, Puneet Srivastav, Satendra Kumar, Vikas Phagna, Lokesh Kumar, Vishal Gupta, Pallavi Kshetrapal, Savita Singh, Nitya Wadhwa, Ramachandran Thiruvengadam, Sreevatsan Raghavan, Mudita Gosain, Tripti Shrivastava, Sankar Bhattacharyya, Jayanta Bhattacharya, Shailendra Asthana, Shailendra Mani
Abstract
Dengue disease is highly prevalent in tropical and subtropical regions worldwide. However, its pathogenesis is still incompletely understood, particularly in comparison to other endemic viruses. Antibody-dependent enhancement (ADE) is a well-known phenomenon for dengue viruses. Given the recent surge in dengue cases and potential cross-reactivity with SARS-CoV-2 antibodies, this study explores the impact of anti-SARS-CoV-2 antibodies on DENV-2 infection. The study assessed the cross-reactivity of SARS-CoV-2 antibodies with the DENV-2 Virus. Human convalescent plasma samples collected during different waves of COVID-19 and monoclonal and polyclonal antibodies raised against SARS-CoV-2 were examined for their potential to cause ADE of DENV-2 infection using cell-based assays. The study found that anti-SARS-CoV-2 antibodies acquired from natural infection in humans or through experimental immunization in animals were cross-reactive with DENV-2 and had the potential to enhance DENV-2 infection in K562 and U937 cells. In-silico and in-vitro studies indicated a strong interaction between SARS-CoV-2 antibodies and DENV-2 E-protein, providing a molecular basis for these findings. This study is the first to demonstrate that anti-SARS-CoV-2 antibodies can cross-react with DENV-2 and can enhance its infection through ADE. These findings have implications for SARS-CoV-2 vaccine development and deployment strategies in regions where dengue is endemic.
https://www.biorxiv.org/content/10.1101/2023.10.09.557914v1.full.pdf