tetano
Editor, Senior Moderator
RNA
. 2021 Nov 29;rna.078825.121.
doi: 10.1261/rna.078825.121. Online ahead of print.
Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot
Christopher P Jones[SUP] 1 [/SUP], Adrian R Ferre-D'Amare[SUP] 2 [/SUP]
Affiliations
Abstract
SARS-CoV-2 produces two long viral protein precursors from one open reading frame using a highly conserved RNA pseudoknot that enhances programmed -1 ribosomal frameshifting. The 1.3 Å-resolution X-ray structure of the pseudoknot reveals three coaxially stacked helices buttressed by idiosyncratic base triples from loop residues. This structure represents a frameshift-stimulating state that must be deformed by the ribosome, and exhibits base-triple-adjacent pockets that could be targeted by future small-molecule therapeutics.
Keywords: COVID; RNA; X-ray; base triple; near-atomic resolution.
. 2021 Nov 29;rna.078825.121.
doi: 10.1261/rna.078825.121. Online ahead of print.
Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot
Christopher P Jones[SUP] 1 [/SUP], Adrian R Ferre-D'Amare[SUP] 2 [/SUP]
Affiliations
- PMID: 34845084
- DOI: 10.1261/rna.078825.121
Abstract
SARS-CoV-2 produces two long viral protein precursors from one open reading frame using a highly conserved RNA pseudoknot that enhances programmed -1 ribosomal frameshifting. The 1.3 Å-resolution X-ray structure of the pseudoknot reveals three coaxially stacked helices buttressed by idiosyncratic base triples from loop residues. This structure represents a frameshift-stimulating state that must be deformed by the ribosome, and exhibits base-triple-adjacent pockets that could be targeted by future small-molecule therapeutics.
Keywords: COVID; RNA; X-ray; base triple; near-atomic resolution.