tetano
Editor, Senior Moderator
Respir Res
. 2022 Dec 13;23(1):343.
doi: 10.1186/s12931-022-02272-7.
Plasma markers of COVID-19 severity: a pilot study
Julia Beimdiek[SUP] #[/SUP][SUP] 1 [/SUP], Sabina Janciauskiene[SUP] #[/SUP][SUP] 2 [/SUP], Sabine Wrenger[SUP] 3 [/SUP], Sonja Volland[SUP] 4 [/SUP], Adriana Rozy[SUP] 5 [/SUP], Jan Fuge[SUP] 3 [/SUP], Beata Olejnicka[SUP] 3 [/SUP], Isabell Pink[SUP] 3 [/SUP], Thomas Illig[SUP] 4 [/SUP], Alexander Popov[SUP] 4 [/SUP], Joanna Chorostowska[SUP] 5 [/SUP], Falk F R Buettner[SUP] 1 [/SUP], Tobias Welte[SUP] 3 [/SUP]
Affiliations
Abstract
Background: SARS-CoV-2 infected patients show heterogeneous clinical presentations ranging from mild symptoms to severe respiratory failure and death. Consequently, various markers reflect this wide spectrum of disease presentations.
Methods: Our pilot cohort included moderate (n = 10) and severe (n = 10) COVID-19 patients, and 10 healthy controls. We determined plasma levels of nine acute phase proteins (APPs) by nephelometry, and full-length (M65), caspase-cleaved (M30) cytokeratin 18, and ADAMTS13 (a disintegrin-like and metalloprotease with thrombospondin type-1 motif 13) by ELISA. In addition, we examined whole plasma N-glycosylation by capillary gel electrophoresis coupled to laser-induced fluorescence detection (CGE-LIF).
Results: When compared to controls, COVID-19 patients had significantly lower concentrations of ADAMTS13 and albumin (ALB) but higher M30, M65, α1-acid glycoprotein (AGP), α1-antitrypsin (AAT), ceruloplasmin (CP), haptoglobin (HP), and high-sensitivity C-reactive protein (hs-CRP). The concentrations of α1-antichymotrypsin (ACT), α2-macroglobulin (A2MG) and serum amyloid A (SAA) proteins did not differ. We found significantly higher levels of AAT and M65 but lower ALB in severe compared to moderate COVID-19 patients. N-glycan analysis of the serum proteome revealed increased levels of oligomannose- and sialylated di-antennary glycans and decreased non-sialylated di-antennary glycan A2G2 in COVID-19 patients compared to controls.
Conclusions: COVID-19-associated changes in levels and N-glycosylation of specific plasma proteins highlight complexity of inflammatory process and grant further investigations.
Keywords: Acute phase proteins; COVID-9 severity; Cell death; Inflammation; N-glycosylation; Trombosis.
. 2022 Dec 13;23(1):343.
doi: 10.1186/s12931-022-02272-7.
Plasma markers of COVID-19 severity: a pilot study
Julia Beimdiek[SUP] #[/SUP][SUP] 1 [/SUP], Sabina Janciauskiene[SUP] #[/SUP][SUP] 2 [/SUP], Sabine Wrenger[SUP] 3 [/SUP], Sonja Volland[SUP] 4 [/SUP], Adriana Rozy[SUP] 5 [/SUP], Jan Fuge[SUP] 3 [/SUP], Beata Olejnicka[SUP] 3 [/SUP], Isabell Pink[SUP] 3 [/SUP], Thomas Illig[SUP] 4 [/SUP], Alexander Popov[SUP] 4 [/SUP], Joanna Chorostowska[SUP] 5 [/SUP], Falk F R Buettner[SUP] 1 [/SUP], Tobias Welte[SUP] 3 [/SUP]
Affiliations
- PMID: 36514048
- DOI: 10.1186/s12931-022-02272-7
Abstract
Background: SARS-CoV-2 infected patients show heterogeneous clinical presentations ranging from mild symptoms to severe respiratory failure and death. Consequently, various markers reflect this wide spectrum of disease presentations.
Methods: Our pilot cohort included moderate (n = 10) and severe (n = 10) COVID-19 patients, and 10 healthy controls. We determined plasma levels of nine acute phase proteins (APPs) by nephelometry, and full-length (M65), caspase-cleaved (M30) cytokeratin 18, and ADAMTS13 (a disintegrin-like and metalloprotease with thrombospondin type-1 motif 13) by ELISA. In addition, we examined whole plasma N-glycosylation by capillary gel electrophoresis coupled to laser-induced fluorescence detection (CGE-LIF).
Results: When compared to controls, COVID-19 patients had significantly lower concentrations of ADAMTS13 and albumin (ALB) but higher M30, M65, α1-acid glycoprotein (AGP), α1-antitrypsin (AAT), ceruloplasmin (CP), haptoglobin (HP), and high-sensitivity C-reactive protein (hs-CRP). The concentrations of α1-antichymotrypsin (ACT), α2-macroglobulin (A2MG) and serum amyloid A (SAA) proteins did not differ. We found significantly higher levels of AAT and M65 but lower ALB in severe compared to moderate COVID-19 patients. N-glycan analysis of the serum proteome revealed increased levels of oligomannose- and sialylated di-antennary glycans and decreased non-sialylated di-antennary glycan A2G2 in COVID-19 patients compared to controls.
Conclusions: COVID-19-associated changes in levels and N-glycosylation of specific plasma proteins highlight complexity of inflammatory process and grant further investigations.
Keywords: Acute phase proteins; COVID-9 severity; Cell death; Inflammation; N-glycosylation; Trombosis.