tetano
Editor, Senior Moderator
Vet J. 2013 Sep 21. pii: S1090-0233(13)00447-4. doi: 10.1016/j.tvjl.2013.09.019. [Epub ahead of print]
Resistance to influenza A virus infection in transformed cell lines expressing an anti-PB2 monoclonal antibody.
Fujimoto Y, Ozaki K, Maeda M, Nishijima KI, Takakuwa H, Otsuki K, Kida H, Ono E.
Source
Department of Biomedicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. Electronic address: yosikazu@anim.med.kyushu-u.ac.jp.
Abstract
The polymerase basic 2 (PB2) protein is one of four proteins that make up the influenza A virus replication complex, which is responsible for viral gene transcription and replication. To assess the antiviral potential of an anti-PB2 monoclonal antibody that inhibits RNA transcription of influenza A viruses, Mardin-Darby canine kidney (MDCK) cells were transformed with two transgenes that encode the light and heavy chains of the monoclonal antibody. The transformed cell lines expressing this monoclonal antibody displayed resistance to several subtypes of influenza A virus infection. In the transformed cell lines infected with influenza A virus, the level of viral RNA transcription was decreased and the effective nuclear transportation of the PB2 protein was also inhibited. These results demonstrate that the anti-PB2 intrabody is potentially able to interfere with the effective nuclear transportation of PB2 protein, resulting in the observed resistance to influenza A virus infection in vitro.
Copyright ? 2013 Elsevier Ltd. All rights reserved.
KEYWORDS:
Antiviral effect, Influenza A virus, Intrabody, PB2 protein, Viral RNA transcription
PMID:
24140339
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24140339
Resistance to influenza A virus infection in transformed cell lines expressing an anti-PB2 monoclonal antibody.
Fujimoto Y, Ozaki K, Maeda M, Nishijima KI, Takakuwa H, Otsuki K, Kida H, Ono E.
Source
Department of Biomedicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. Electronic address: yosikazu@anim.med.kyushu-u.ac.jp.
Abstract
The polymerase basic 2 (PB2) protein is one of four proteins that make up the influenza A virus replication complex, which is responsible for viral gene transcription and replication. To assess the antiviral potential of an anti-PB2 monoclonal antibody that inhibits RNA transcription of influenza A viruses, Mardin-Darby canine kidney (MDCK) cells were transformed with two transgenes that encode the light and heavy chains of the monoclonal antibody. The transformed cell lines expressing this monoclonal antibody displayed resistance to several subtypes of influenza A virus infection. In the transformed cell lines infected with influenza A virus, the level of viral RNA transcription was decreased and the effective nuclear transportation of the PB2 protein was also inhibited. These results demonstrate that the anti-PB2 intrabody is potentially able to interfere with the effective nuclear transportation of PB2 protein, resulting in the observed resistance to influenza A virus infection in vitro.
Copyright ? 2013 Elsevier Ltd. All rights reserved.
KEYWORDS:
Antiviral effect, Influenza A virus, Intrabody, PB2 protein, Viral RNA transcription
PMID:
24140339
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24140339