tetano
Editor, Senior Moderator
J Virol. 2012 Feb 15. [Epub ahead of print]
Receptor-binding Profiles of H7 Subtype Influenza Viruses in Different Host Species.
Gambaryan AS, Matrosovich TY, Philipp J, Munster VJ, Fouchier RA, Cattoli G, Capua I, Krauss SL, Webster RG, Banks J, Bovin NV, Klenk HD, Matrosovich MN.
Source
M.P.Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, 142782 Moscow, Russia.
Abstract
Influenza viruses of gallinaceous poultry and wild aquatic birds usually have distinguishable receptor-binding properties. Here we used a panel of synthetic sialylglycopolymers and solid-phase receptor-binding assays to characterize receptor-binding profiles of about 70 H7 influenza viruses isolated from aquatic birds, land-based poultry and horses in Eurasia and America. Unlike typical duck viruses with non-H7 HA, all avian H7 viruses irrespective of the host species displayed a poultry-virus-like binding specificity, i.e., preferential binding to sulfated oligosaccharides Neu5Acα2-3Galβ1-4(6-O-HSO(3))GlcNAc and Neu5Acα2-3Galβ1-4(Fucα1-3)(6-O-HSO(3))GlcNAc. This phenotype correlated with the unique amino acid sequence of the 185-189 loop of H7 HA and seemed to be dependent on ionic interactions between the sulfate group of the receptor and Lys193 and on the lack of sterical clashes between the fucose residue and Gln222. Many North American and Eurasian H7 viruses displayed weak but detectable binding to human-type receptor moiety Neu5Acα2-6Galβ1-4GlcNAc highlighting the potential of H7 viruses for avian-to-human transmission. Equine H7 viruses differed from other viruses by preferential binding to the N-glycolyl form of sialic acid. Our data suggest that the receptor-binding site of contemporary H7 viruses in aquatic and terrestrial birds has been formed after the introduction of their common precursor from ducks to a new host, presumably, gallinaceous poultry. The uniformity of receptor-binding profile of H7 viruses in various wild and domestic birds indicates that there is no strong receptor-mediated host range restriction in birds on viruses with this HA subtype. This notion agrees with repeated interspecies transmission of H7 viruses from aquatic birds to poultry.
PMID:
22345462
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22345462
Receptor-binding Profiles of H7 Subtype Influenza Viruses in Different Host Species.
Gambaryan AS, Matrosovich TY, Philipp J, Munster VJ, Fouchier RA, Cattoli G, Capua I, Krauss SL, Webster RG, Banks J, Bovin NV, Klenk HD, Matrosovich MN.
Source
M.P.Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, 142782 Moscow, Russia.
Abstract
Influenza viruses of gallinaceous poultry and wild aquatic birds usually have distinguishable receptor-binding properties. Here we used a panel of synthetic sialylglycopolymers and solid-phase receptor-binding assays to characterize receptor-binding profiles of about 70 H7 influenza viruses isolated from aquatic birds, land-based poultry and horses in Eurasia and America. Unlike typical duck viruses with non-H7 HA, all avian H7 viruses irrespective of the host species displayed a poultry-virus-like binding specificity, i.e., preferential binding to sulfated oligosaccharides Neu5Acα2-3Galβ1-4(6-O-HSO(3))GlcNAc and Neu5Acα2-3Galβ1-4(Fucα1-3)(6-O-HSO(3))GlcNAc. This phenotype correlated with the unique amino acid sequence of the 185-189 loop of H7 HA and seemed to be dependent on ionic interactions between the sulfate group of the receptor and Lys193 and on the lack of sterical clashes between the fucose residue and Gln222. Many North American and Eurasian H7 viruses displayed weak but detectable binding to human-type receptor moiety Neu5Acα2-6Galβ1-4GlcNAc highlighting the potential of H7 viruses for avian-to-human transmission. Equine H7 viruses differed from other viruses by preferential binding to the N-glycolyl form of sialic acid. Our data suggest that the receptor-binding site of contemporary H7 viruses in aquatic and terrestrial birds has been formed after the introduction of their common precursor from ducks to a new host, presumably, gallinaceous poultry. The uniformity of receptor-binding profile of H7 viruses in various wild and domestic birds indicates that there is no strong receptor-mediated host range restriction in birds on viruses with this HA subtype. This notion agrees with repeated interspecies transmission of H7 viruses from aquatic birds to poultry.
PMID:
22345462
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22345462