Realtime RT-PCR Assay Unable to Detect H7 Subtype Avian Influenza Viruses Isolated from Wild Birds

[Sally Furniss]

Realtime RT-PCR Assay Unable to Detect H7 Subtype Avian Influenza Viruses Isolated from Wild Birds

<nobr>Zheng Xing^*,</nobr> <nobr>Carol Cardona,</nobr> <nobr>Phuong Dao,</nobr> <nobr>Beate Crossley,</nobr> <nobr>Sharon Hietala,</nobr> and <nobr>Walter Boyce</nobr> Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; California Animal Health and Food Safety Laboratory; Wildlife Health Center


[SIZE=-1] ^* To whom correspondence should be addressed. Email: zxing@ucdavis.edu<script type="text/javascript"><!-- var u = "zxing", d = "ucdavis.edu"; document.getElementById("em0").innerHTML = '<a href="mailto:' + u + '@' + d + '">' + u + '@' + d + '<\/a>'//--></script>. [/SIZE]
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</td> <th align="left" valign="middle" width="95%">[SIZE=+2] Abstract[/SIZE]</th></tr></tbody></table>
We report a failure of the current real-time RT-PCR H7 subtypingprotocol, used in national avian influenza surveillance programs.Significant substitutions in primer and probe target sequenceswere identified, especially in wild bird viruses. The protocol,designed originally for detecting H7 influenza viruses in poultry,is not reliable for wild bird surveillance.

http://jcm.asm.org/cgi/content/abstract/JCM.02426-07v1

 

[HenryN]

Re: Realtime RT-PCR Assay Unable to Detect H7 Subtype Avian Influenza Viruses Isolated from Wild Birds

Realtime RT-PCR Assay Unable to Detect H7 Subtype Avian Influenza Viruses Isolated from Wild Birds

<NOBR>Zheng Xing<SUP>*</SUP>,</NOBR> <NOBR>Carol Cardona,</NOBR> <NOBR>Phuong Dao,</NOBR> <NOBR>Beate Crossley,</NOBR> <NOBR>Sharon Hietala,</NOBR> and <NOBR>Walter Boyce</NOBR> Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; California Animal Health and Food Safety Laboratory; Wildlife Health Center


[SIZE=-1]<SUP>*</SUP> To whom correspondence should be addressed. Email: zxing@ucdavis.edu<SCRIPT type=text/javascript><!-- var u = "zxing", d = "ucdavis.edu"; document.getElementById("em0").innerHTML = '<a href="mailto:' + u + '@' + d + '">' + u + '@' + d + '<\/a>'//--></SCRIPT>. [/SIZE]
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<TABLE cellSpacing=0 cellPadding=0 width="100%" bgColor=#e1e1e1><TBODY><TR><TD vAlign=center align=left width="5%" bgColor=#ffffff>

</TD><TH vAlign=center align=left width="95%">[SIZE=+2]Abstract[/SIZE]</TH></TR></TBODY></TABLE>
We report a failure of the current real-time RT-PCR H7 subtyping<SUP> </SUP>protocol, used in national avian influenza surveillance programs.<SUP> </SUP>Significant substitutions in primer and probe target sequences<SUP> </SUP>were identified, especially in wild bird viruses. The protocol,<SUP> </SUP>designed originally for detecting H7 influenza viruses in poultry,<SUP> </SUP>is not reliable for wild bird surveillance.

http://jcm.asm.org/cgi/content/abstract/JCM.02426-07v1

More evidence of an abysmal surveillance program (worldwide).