tetano
Editor, Senior Moderator
J Sep Sci. 2016 Apr 8. doi: 10.1002/jssc.201600087. [Epub ahead of print]
[h=1]Rapid screening, identification and purification of neuraminidase inhibitors from Lithospermum erythrorhizon Sieb.et Zucc. by ultrafiltration with HPLC-ESI-TOF-MS combined with semi-preparative HPLC.[/h] Zhang M[SUP]1,[/SUP][SUP]2[/SUP], Zhao H[SUP]1[/SUP], Zhao Z[SUP]1[/SUP], Yan H[SUP]1[/SUP], Lv R[SUP]1[/SUP], Cui L[SUP]1[/SUP], Yuan J[SUP]1[/SUP], Wang D[SUP]1[/SUP], Geng Y[SUP]1[/SUP], Liu D[SUP]2[/SUP], Wang X[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We put forward an efficient strategy based on bioassay guidance for the rapid screening, identification and purification of the neuraminidase inhibitors from traditional Chinese medicines, and apply to the discovery of anti-influenza components from Lithospermiun erythrorhizon Sieb.et Zucc. Ultrafiltration with high-performance liquid chromatography and electrospray ionization time-of-flight mass spectrometry was employed for the rapid screening and preliminarily identification of anti-influenza components from Zicao. Semi-preparative high-performance liquid chromatography was used for the rapid separation and purification of the target compounds. NMR spectroscopy, mass spectrometry and UV spectroscopy was used for further structural identification, and the activity of the compounds was verified by in vitro assay. Five compounds were found to have neuraminidase inhibitory activity by this method. Subsequently, the five compounds were separated by semi-preparative high-performance liquid chromatography with the purity over 98% for all of them by high-performance liquid chromatography test. Combined with the NMR spectroscopy, mass spectrometry and UV spectroscopy data, they were identified as alkannin, acetyl alkannin, isobutyryl alkannin, β,β-dimethylacryloyl alkannin and isovaleryl alkannin. The in vitro assay showed that all five compounds had good neuraminidase inhibitory activities. These results suggested that the method highly efficient, and it can provide platform and methodology supports for the rapid discovery of anti-influenza active ingredients from complex Chinese herbal medicines. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] High-performance liquid chromatography;Lithospermiun erythrorhizon Sieb.et Zucc.; neuraminidase inhibitor; semi-preparative High-performance liquid chromatography; ultrafiltration
PMID: 27061885 [PubMed - as supplied by publisher]
[h=1]Rapid screening, identification and purification of neuraminidase inhibitors from Lithospermum erythrorhizon Sieb.et Zucc. by ultrafiltration with HPLC-ESI-TOF-MS combined with semi-preparative HPLC.[/h] Zhang M[SUP]1,[/SUP][SUP]2[/SUP], Zhao H[SUP]1[/SUP], Zhao Z[SUP]1[/SUP], Yan H[SUP]1[/SUP], Lv R[SUP]1[/SUP], Cui L[SUP]1[/SUP], Yuan J[SUP]1[/SUP], Wang D[SUP]1[/SUP], Geng Y[SUP]1[/SUP], Liu D[SUP]2[/SUP], Wang X[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We put forward an efficient strategy based on bioassay guidance for the rapid screening, identification and purification of the neuraminidase inhibitors from traditional Chinese medicines, and apply to the discovery of anti-influenza components from Lithospermiun erythrorhizon Sieb.et Zucc. Ultrafiltration with high-performance liquid chromatography and electrospray ionization time-of-flight mass spectrometry was employed for the rapid screening and preliminarily identification of anti-influenza components from Zicao. Semi-preparative high-performance liquid chromatography was used for the rapid separation and purification of the target compounds. NMR spectroscopy, mass spectrometry and UV spectroscopy was used for further structural identification, and the activity of the compounds was verified by in vitro assay. Five compounds were found to have neuraminidase inhibitory activity by this method. Subsequently, the five compounds were separated by semi-preparative high-performance liquid chromatography with the purity over 98% for all of them by high-performance liquid chromatography test. Combined with the NMR spectroscopy, mass spectrometry and UV spectroscopy data, they were identified as alkannin, acetyl alkannin, isobutyryl alkannin, β,β-dimethylacryloyl alkannin and isovaleryl alkannin. The in vitro assay showed that all five compounds had good neuraminidase inhibitory activities. These results suggested that the method highly efficient, and it can provide platform and methodology supports for the rapid discovery of anti-influenza active ingredients from complex Chinese herbal medicines. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] High-performance liquid chromatography;Lithospermiun erythrorhizon Sieb.et Zucc.; neuraminidase inhibitor; semi-preparative High-performance liquid chromatography; ultrafiltration
PMID: 27061885 [PubMed - as supplied by publisher]