tetano
Editor, Senior Moderator
Small. 2015 Aug 17. doi: 10.1002/smll.201403746. [Epub ahead of print]
[h=1]Rapid and Quantitative Detection of Avian Influenza A(H7N9) Virions in Complex Matrices Based on Combined Magnetic Capture and Quantum Dot Labeling.[/h] Wu M[SUP]1[/SUP], Zhang ZL[SUP]1[/SUP], Chen G[SUP]1[/SUP], Wen CY[SUP]1[/SUP], Wu LL[SUP]1[/SUP], Hu J[SUP]1[/SUP], Xiong CC[SUP]2[/SUP], Chen JJ[SUP]2[/SUP], Pang DW[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Avian influenza A(H7N9) virus, which emerged in China in the spring of 2013, has infected hundreds of people and resulted in many deaths. Herein, a rapid and quantitative assay is proposed for the one-step detection of H7N9 virions. Immunomagnetic nanospheres (IMNs) and antibody-conjugated quantum dots (Ab-QDs) are simultaneously employed to capture and identify the target virus, leading to a high efficiency, good specificity, and strong anti-interference ability. Moreover, this reliable detection assay, which combines the efficient magnetic enrichment and the unique photophysical properties of QDs, can achieve a high sensitivity for a low detection limit. At the same time, this detection strategy shows great flexibility for employment in a variety of fluorescence detectors, including fluorescence spectrometry, microscope assays, and handheld UV lamp tests. Furthermore, our one-step detection strategy induces very little change in the integrity of the vulnerable virions, which enables additional genotyping testing following the fluorescence detection. The present study, thus, reports a rapid and quantitative approach for the detection of H7N9 virions based on simultaneous magnetic capture and QD labeling, thereby providing a higher probability for detection and therefore faster diagnosis of H7N9-infected patients.
? 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
[h=4]KEYWORDS:[/h] detectors; magnetic nanospheres; quantum dots; sensors; viral infections, avian influenza
PMID: 26280101 [PubMed - as supplied by publisher]
[h=1]Rapid and Quantitative Detection of Avian Influenza A(H7N9) Virions in Complex Matrices Based on Combined Magnetic Capture and Quantum Dot Labeling.[/h] Wu M[SUP]1[/SUP], Zhang ZL[SUP]1[/SUP], Chen G[SUP]1[/SUP], Wen CY[SUP]1[/SUP], Wu LL[SUP]1[/SUP], Hu J[SUP]1[/SUP], Xiong CC[SUP]2[/SUP], Chen JJ[SUP]2[/SUP], Pang DW[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Avian influenza A(H7N9) virus, which emerged in China in the spring of 2013, has infected hundreds of people and resulted in many deaths. Herein, a rapid and quantitative assay is proposed for the one-step detection of H7N9 virions. Immunomagnetic nanospheres (IMNs) and antibody-conjugated quantum dots (Ab-QDs) are simultaneously employed to capture and identify the target virus, leading to a high efficiency, good specificity, and strong anti-interference ability. Moreover, this reliable detection assay, which combines the efficient magnetic enrichment and the unique photophysical properties of QDs, can achieve a high sensitivity for a low detection limit. At the same time, this detection strategy shows great flexibility for employment in a variety of fluorescence detectors, including fluorescence spectrometry, microscope assays, and handheld UV lamp tests. Furthermore, our one-step detection strategy induces very little change in the integrity of the vulnerable virions, which enables additional genotyping testing following the fluorescence detection. The present study, thus, reports a rapid and quantitative approach for the detection of H7N9 virions based on simultaneous magnetic capture and QD labeling, thereby providing a higher probability for detection and therefore faster diagnosis of H7N9-infected patients.
? 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
[h=4]KEYWORDS:[/h] detectors; magnetic nanospheres; quantum dots; sensors; viral infections, avian influenza
PMID: 26280101 [PubMed - as supplied by publisher]