tetano
Editor, Senior Moderator
Public Health
. 2020 Jul 10;186:1-5.
doi: 10.1016/j.puhe.2020.07.011. Online ahead of print.
Diagnostic value of combined nucleic acid and antibody detection in suspected COVID-19 cases
H Zeng[SUP] 1 [/SUP], S Deng[SUP] 2 [/SUP], Z Zhou[SUP] 3 [/SUP], X Qiu[SUP] 4 [/SUP], X Jia[SUP] 5 [/SUP], Z Li[SUP] 1 [/SUP], J Wang[SUP] 1 [/SUP], H Duan[SUP] 1 [/SUP], L Tu[SUP] 1 [/SUP], J Wang[SUP] 6 [/SUP]
Affiliations
Abstract
Objectives: Nucleic acid testing is the gold standard method for the diagnosis of coronavirus disease 2019 (COVID-19); however, large numbers of false-negative results have been reported. In this study, nucleic acid detection and antibody detection (IgG and IgM) were combined to improve the testing accuracy of patients with suspected COVID-19.
Study design: The positive rate of nucleic acid detection and antibody detection (IgG and IgM) were compared in suspected COVID-19 patients.
Methods: A total of 71 patients with suspected COVID-19 were selected to participate in this study, which included a retrospective analysis of clinical features, imaging examination, laboratory biochemical examination and nucleic acid detection and specific antibody (IgM and IgG) detection.
Results: The majority of participants with suspected COVID-19 presented with fever (67.61%) and cough (54.93%), and the imaging results showed multiple small patches and ground-glass opacity in both lungs, with less common infiltration and consolidation opacity (23.94%). Routine blood tests were mostly normal (69.01%), although only a few patients had lymphopenia (4.23%) or leucopenia (12.68%). There was no statistical difference in the double-positive rate between nucleic acid detection (46.48%) and specific antibody (IgG and IgM) detection (42.25%) (P = 0.612), both of which were also poorly consistent with each other (kappa = 0.231). The positive rate of combined nucleic acid detection and antibody detection (63.38%) was significantly increased, compared with that of nucleic acid detection (46.48%) and that of specific antibody (IgG and IgM) detection (42.25%), and the differences were statistically significant (P = 0.043 and P = 0.012, respectively).
Conclusions: Nucleic acid detection and specific antibody (IgG and IgM) detection had similar positive rates, and their combination could improve the positive rate of COVID-19 detection, which is of great significance for diagnosis and epidemic control.
Keywords: IgG and IgM antibody detection; Novel coronavirus infection; Nucleic acid detection; Suspected cases.
. 2020 Jul 10;186:1-5.
doi: 10.1016/j.puhe.2020.07.011. Online ahead of print.
Diagnostic value of combined nucleic acid and antibody detection in suspected COVID-19 cases
H Zeng[SUP] 1 [/SUP], S Deng[SUP] 2 [/SUP], Z Zhou[SUP] 3 [/SUP], X Qiu[SUP] 4 [/SUP], X Jia[SUP] 5 [/SUP], Z Li[SUP] 1 [/SUP], J Wang[SUP] 1 [/SUP], H Duan[SUP] 1 [/SUP], L Tu[SUP] 1 [/SUP], J Wang[SUP] 6 [/SUP]
Affiliations
- PMID: 32731151
- PMCID: PMC7351380
- DOI: 10.1016/j.puhe.2020.07.011
Abstract
Objectives: Nucleic acid testing is the gold standard method for the diagnosis of coronavirus disease 2019 (COVID-19); however, large numbers of false-negative results have been reported. In this study, nucleic acid detection and antibody detection (IgG and IgM) were combined to improve the testing accuracy of patients with suspected COVID-19.
Study design: The positive rate of nucleic acid detection and antibody detection (IgG and IgM) were compared in suspected COVID-19 patients.
Methods: A total of 71 patients with suspected COVID-19 were selected to participate in this study, which included a retrospective analysis of clinical features, imaging examination, laboratory biochemical examination and nucleic acid detection and specific antibody (IgM and IgG) detection.
Results: The majority of participants with suspected COVID-19 presented with fever (67.61%) and cough (54.93%), and the imaging results showed multiple small patches and ground-glass opacity in both lungs, with less common infiltration and consolidation opacity (23.94%). Routine blood tests were mostly normal (69.01%), although only a few patients had lymphopenia (4.23%) or leucopenia (12.68%). There was no statistical difference in the double-positive rate between nucleic acid detection (46.48%) and specific antibody (IgG and IgM) detection (42.25%) (P = 0.612), both of which were also poorly consistent with each other (kappa = 0.231). The positive rate of combined nucleic acid detection and antibody detection (63.38%) was significantly increased, compared with that of nucleic acid detection (46.48%) and that of specific antibody (IgG and IgM) detection (42.25%), and the differences were statistically significant (P = 0.043 and P = 0.012, respectively).
Conclusions: Nucleic acid detection and specific antibody (IgG and IgM) detection had similar positive rates, and their combination could improve the positive rate of COVID-19 detection, which is of great significance for diagnosis and epidemic control.
Keywords: IgG and IgM antibody detection; Novel coronavirus infection; Nucleic acid detection; Suspected cases.