tetano
Editor, Senior Moderator
Int Immunopharmacol. 2014 Sep 1. pii: S1567-5769(14)00333-6. doi: 10.1016/j.intimp.2014.08.019. [Epub ahead of print]
Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab')2.
Zhao Z1, Fan C2, Duan Y1, Zhang L1, Li M3, Yang X1, Li R4, Yang P4, Wang X5.
Author information
Abstract
Influenza A H7N9 virus has demonstrated considerable pandemic potential in China ever since early spring 2013. Until now, there have been no specific medicines to treat influenza A H7N9 virus infected patients. Development of a safe and effective H7N9 therapeutic preparation is urgently needed. To this end, we prepared and evaluated the pepsin-digested F(ab')2 fragments of serum IgGs from the horses inoculated with a inactivated influenza A H7N9 whole virus antigens. The protective effects of the F(ab')2 fragments against H7N9 virus infection were determined in cultured MDCK cells by cytopathic effect (CPE) and evaluated in a BALB/c mouse model by observing death, weight loss and viral load. The in vitro results showed that the F(ab')2 fragments had an HI titer of 1:2048 and a neutralization titer of 1: 31,623. The in vivo assays suggested that 600U of the preparations could efficiently protect BALB/c mice from a lethal dose of A/Anhui/01/2013 (H7N9) infection even when administered two days post infection. Thus, this highly purified preparation should be a potential candidate for treating severe patients suffering from influenza A H7N9.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
Equine immunoglobulin F(ab′)(2) fragments; H7N9
PMID:
25192652
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25192652
Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab')2.
Zhao Z1, Fan C2, Duan Y1, Zhang L1, Li M3, Yang X1, Li R4, Yang P4, Wang X5.
Author information
Abstract
Influenza A H7N9 virus has demonstrated considerable pandemic potential in China ever since early spring 2013. Until now, there have been no specific medicines to treat influenza A H7N9 virus infected patients. Development of a safe and effective H7N9 therapeutic preparation is urgently needed. To this end, we prepared and evaluated the pepsin-digested F(ab')2 fragments of serum IgGs from the horses inoculated with a inactivated influenza A H7N9 whole virus antigens. The protective effects of the F(ab')2 fragments against H7N9 virus infection were determined in cultured MDCK cells by cytopathic effect (CPE) and evaluated in a BALB/c mouse model by observing death, weight loss and viral load. The in vitro results showed that the F(ab')2 fragments had an HI titer of 1:2048 and a neutralization titer of 1: 31,623. The in vivo assays suggested that 600U of the preparations could efficiently protect BALB/c mice from a lethal dose of A/Anhui/01/2013 (H7N9) infection even when administered two days post infection. Thus, this highly purified preparation should be a potential candidate for treating severe patients suffering from influenza A H7N9.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
Equine immunoglobulin F(ab′)(2) fragments; H7N9
PMID:
25192652
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25192652