tetano
Editor, Senior Moderator
J Virol Methods. 2015 Feb 11. pii: S0166-0934(15)00017-8. doi: 10.1016/j.jviromet.2015.01.002. [Epub ahead of print]
[h=1]Prospective comparison of RT-PCR/ESI-MS to Prodesse ProFlu Plus and Cepheid GenXpert for the detection of Influenza A and B viruses.[/h] Hardick J[SUP]1[/SUP], Dugas A[SUP]2[/SUP], Goheen J[SUP]3[/SUP], Rothman R[SUP]2[/SUP], Gaydos C[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] RT-PCR/ESI-MS has previously demonstrated the capability to detect and identify respiratory viral pathogens in nasopharyngeal swabs. This study expands on previous research by performing a prospective evaluation of RT-PCR/ESI-MS to detect and identify Influenza A and B viruses compared to Prodesse ProFlu Plus and combined ProFlu Plus and Cepheid Xpert Flu. ProFlu Plus was also used as a gold standard for comparison for respiratory syncytial virus detection. Using ProFlu Plus as a gold standard, RT-PCR/ESI-MS had sensitivity and specificity of 82.1% (23/28) and 100% (258/258), respectively, for Influenza A, 100% (16/16) and 99.6% (269/270), respectively for Influenza B, and 88.6% (39/44) and 99.6% (241/242) for any Influenza virus. Using matching results from ProFlu Plus and Xpert Flu as a gold standard, RT-PCR/ESI-MS had 85.2% (23/27) and 100% (259/259) sensitivity and specificity respectively for Influenza A, 100% (14/14) and 99.6% (270/272), respectively for Influenza B virus. Overall, RT-PCR/ESI-MS was not as sensitive as the combined gold standard of ProFlu Plus and Xpert Flu, although it has the capability of detecting other respiratory viruses.
Copyright ? 2015. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Influenza virus; Molecular diagnostics; RT-PCR/ESI-MS; Respiratory viruses
PMID: 25681525 [PubMed - as supplied by publisher]
[h=1]Prospective comparison of RT-PCR/ESI-MS to Prodesse ProFlu Plus and Cepheid GenXpert for the detection of Influenza A and B viruses.[/h] Hardick J[SUP]1[/SUP], Dugas A[SUP]2[/SUP], Goheen J[SUP]3[/SUP], Rothman R[SUP]2[/SUP], Gaydos C[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] RT-PCR/ESI-MS has previously demonstrated the capability to detect and identify respiratory viral pathogens in nasopharyngeal swabs. This study expands on previous research by performing a prospective evaluation of RT-PCR/ESI-MS to detect and identify Influenza A and B viruses compared to Prodesse ProFlu Plus and combined ProFlu Plus and Cepheid Xpert Flu. ProFlu Plus was also used as a gold standard for comparison for respiratory syncytial virus detection. Using ProFlu Plus as a gold standard, RT-PCR/ESI-MS had sensitivity and specificity of 82.1% (23/28) and 100% (258/258), respectively, for Influenza A, 100% (16/16) and 99.6% (269/270), respectively for Influenza B, and 88.6% (39/44) and 99.6% (241/242) for any Influenza virus. Using matching results from ProFlu Plus and Xpert Flu as a gold standard, RT-PCR/ESI-MS had 85.2% (23/27) and 100% (259/259) sensitivity and specificity respectively for Influenza A, 100% (14/14) and 99.6% (270/272), respectively for Influenza B virus. Overall, RT-PCR/ESI-MS was not as sensitive as the combined gold standard of ProFlu Plus and Xpert Flu, although it has the capability of detecting other respiratory viruses.
Copyright ? 2015. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Influenza virus; Molecular diagnostics; RT-PCR/ESI-MS; Respiratory viruses
PMID: 25681525 [PubMed - as supplied by publisher]