tetano
Editor, Senior Moderator
J Gen Virol. 2015 Dec 2. doi: 10.1099/jgv.0.000357. [Epub ahead of print]
[h=1]Proinflammatory Cytokine Dysregulation is Associated with Novel Avian Influenza A (H7N9) Virus in Primary Human Macrophages.[/h] Zhao C[SUP]1[/SUP], Qi X[SUP]2[/SUP], Ding M[SUP]3[/SUP], Sun X[SUP]4[/SUP], Zhou Z[SUP]5[/SUP], Zhang S[SUP]6[/SUP], Zen K[SUP]7[/SUP], Li X[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Since March 2013, more than 500 laboratory-confirmed human H7N9 influenza A virus infection cases have been recorded, with a case-fatality rate of more than 30%. Clinical research has shown that cytokine and chemokine dysregulation contributes to the pathogenicity of the H7N9 virus. Here, we investigated cytokine profiles in primary human macrophages infected with the novel H7N9 virus, using cytokine antibody arrays. The levels of several proinflammatory cytokines, particularly tumour necrosis factor α (TNF-α), were increased in H7N9-infected macrophages. The induction of the transcriptional and translational levels of the proinflammatory cytokines by H7N9 virus seemed to be intermediate between those induced by highly pathogenic avian H5N1 and pandemic human H1N1 viruses, which were detected by ELISA and RT-qPCR, respectively. Additionally, compared with H5N1, the upregulation of proinflammatory cytokines caused by H7N9 infection occurred rapidly but mildly. Our results identified the overall profiles of cytokine and chemokine induction by the H7N9 influenza virus in an in vitro cell culture model, and they could provide potential therapeutic targets for the control of severe human H7N9 disease.
PMID: 26644088 [PubMed - as supplied by publisher]
[h=1]Proinflammatory Cytokine Dysregulation is Associated with Novel Avian Influenza A (H7N9) Virus in Primary Human Macrophages.[/h] Zhao C[SUP]1[/SUP], Qi X[SUP]2[/SUP], Ding M[SUP]3[/SUP], Sun X[SUP]4[/SUP], Zhou Z[SUP]5[/SUP], Zhang S[SUP]6[/SUP], Zen K[SUP]7[/SUP], Li X[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Since March 2013, more than 500 laboratory-confirmed human H7N9 influenza A virus infection cases have been recorded, with a case-fatality rate of more than 30%. Clinical research has shown that cytokine and chemokine dysregulation contributes to the pathogenicity of the H7N9 virus. Here, we investigated cytokine profiles in primary human macrophages infected with the novel H7N9 virus, using cytokine antibody arrays. The levels of several proinflammatory cytokines, particularly tumour necrosis factor α (TNF-α), were increased in H7N9-infected macrophages. The induction of the transcriptional and translational levels of the proinflammatory cytokines by H7N9 virus seemed to be intermediate between those induced by highly pathogenic avian H5N1 and pandemic human H1N1 viruses, which were detected by ELISA and RT-qPCR, respectively. Additionally, compared with H5N1, the upregulation of proinflammatory cytokines caused by H7N9 infection occurred rapidly but mildly. Our results identified the overall profiles of cytokine and chemokine induction by the H7N9 influenza virus in an in vitro cell culture model, and they could provide potential therapeutic targets for the control of severe human H7N9 disease.
PMID: 26644088 [PubMed - as supplied by publisher]