tetano
Editor, Senior Moderator
Methods Mol Biol. 2020;2132:567-583. doi: 10.1007/978-1-0716-0430-4_49.
Preparation and Detection of Glycan-Binding Activity of Influenza Virus.
Nakakita SI[SUP]1[/SUP], Sriwilaijaroen N[SUP]2[/SUP], Suzuki Y[SUP]3[/SUP], Hirabayashi J[SUP]4[/SUP].
Author information
Abstract
We describe a method to detect influenza virus using an evanescent-field-activated fluorescence scanner type glycan array and ELISA system. Neoglycoprotein was prepared by combination of organic chemistry and biomaterial preparation. These ligands were spotted on a glass plate or plastic well to make a glycan array and ELISA plate. We detected cultured influenza virus using glycan array and ELISA. Then, we showed that the neoglycoprotein binds to Cy3-labeled hemagglutinins (H1 and H5), a NeuAcα2,6LacNAc or NeuAcα2,3LacNAc recognized protein, as detected.
KEYWORDS:
Evanescent-field-activated fluorescence scanner; Glycan array; Hemagglutinin; Influenza virus; Neoglycoprotein
PMID:32306357DOI:10.1007/978-1-0716-0430-4_49
Preparation and Detection of Glycan-Binding Activity of Influenza Virus.
Nakakita SI[SUP]1[/SUP], Sriwilaijaroen N[SUP]2[/SUP], Suzuki Y[SUP]3[/SUP], Hirabayashi J[SUP]4[/SUP].
Author information
Abstract
We describe a method to detect influenza virus using an evanescent-field-activated fluorescence scanner type glycan array and ELISA system. Neoglycoprotein was prepared by combination of organic chemistry and biomaterial preparation. These ligands were spotted on a glass plate or plastic well to make a glycan array and ELISA plate. We detected cultured influenza virus using glycan array and ELISA. Then, we showed that the neoglycoprotein binds to Cy3-labeled hemagglutinins (H1 and H5), a NeuAcα2,6LacNAc or NeuAcα2,3LacNAc recognized protein, as detected.
KEYWORDS:
Evanescent-field-activated fluorescence scanner; Glycan array; Hemagglutinin; Influenza virus; Neoglycoprotein
PMID:32306357DOI:10.1007/978-1-0716-0430-4_49