tetano
Editor, Senior Moderator
PLoS Pathog
. 2024 Dec 5;20(12):e1012713.
doi: 10.1371/journal.ppat.1012713. eCollection 2024 Dec. Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants
Chunliang Luo[SUP] 1 2 [/SUP], Linjie Li[SUP] 2 3 [/SUP], Yuhang Gu[SUP] 2 4 [/SUP], Hangchuan Zhang[SUP] 1 [/SUP], Zepeng Xu[SUP] 2 5 [/SUP], Junqing Sun[SUP] 1 [/SUP], Kaiyuan Shi[SUP] 6 [/SUP], Sufang Ma[SUP] 2 [/SUP], Wen-Xia Tian[SUP] 1 [/SUP], Kefang Liu[SUP] 2 3 [/SUP], George F Gao[SUP] 1 2 3 [/SUP]
Affiliations
Raccoon dog was proposed as a potential host of SARS-CoV-2, but no evidence support such a notion. In our study, we investigated the binding affinities of raccoon dog ACE2 (rdACE2) to the spike (S) protein receptor binding domain (RBD) of SARS-CoV-2 prototype (PT) and its variants. It revealed that the binding affinities of RBD from SARS-CoV-2 variants were generally lower than that of the PT RBD. Through structural and functional analyses, we found amino acids H34 and M82 play pivotal roles in maintaining the binding affinity of ACE2 to different SARS-CoV-2 sub-variants. These results suggest that raccoon dogs exhibit lower susceptibility to SARS-CoV-2 compared to those animal species with a high prevalence of SARS-CoV-2 transmission.
. 2024 Dec 5;20(12):e1012713.
doi: 10.1371/journal.ppat.1012713. eCollection 2024 Dec. Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants
Chunliang Luo[SUP] 1 2 [/SUP], Linjie Li[SUP] 2 3 [/SUP], Yuhang Gu[SUP] 2 4 [/SUP], Hangchuan Zhang[SUP] 1 [/SUP], Zepeng Xu[SUP] 2 5 [/SUP], Junqing Sun[SUP] 1 [/SUP], Kaiyuan Shi[SUP] 6 [/SUP], Sufang Ma[SUP] 2 [/SUP], Wen-Xia Tian[SUP] 1 [/SUP], Kefang Liu[SUP] 2 3 [/SUP], George F Gao[SUP] 1 2 3 [/SUP]
Affiliations
- PMID: 39637248
- DOI: 10.1371/journal.ppat.1012713
Raccoon dog was proposed as a potential host of SARS-CoV-2, but no evidence support such a notion. In our study, we investigated the binding affinities of raccoon dog ACE2 (rdACE2) to the spike (S) protein receptor binding domain (RBD) of SARS-CoV-2 prototype (PT) and its variants. It revealed that the binding affinities of RBD from SARS-CoV-2 variants were generally lower than that of the PT RBD. Through structural and functional analyses, we found amino acids H34 and M82 play pivotal roles in maintaining the binding affinity of ACE2 to different SARS-CoV-2 sub-variants. These results suggest that raccoon dogs exhibit lower susceptibility to SARS-CoV-2 compared to those animal species with a high prevalence of SARS-CoV-2 transmission.