tetano
Editor, Senior Moderator
PLoS Pathog
. 2021 Sep 23;17(9):e1009566.
doi: 10.1371/journal.ppat.1009566. Online ahead of print.
Characterization of changes in the hemagglutinin that accompanied the emergence of H3N2/1968 pandemic influenza viruses
Johanna West[SUP] 1 [/SUP], Juliane Röder[SUP] 1 [/SUP], Tatyana Matrosovich[SUP] 1 [/SUP], Jana Beicht[SUP] 1 [/SUP], Jan Baumann[SUP] 1 [/SUP], Nancy Mounogou Kouassi[SUP] 1 [/SUP], Jennifer Doedt[SUP] 1 [/SUP], Nicolai Bovin[SUP] 2 [/SUP], Gianpiero Zamperin[SUP] 3 [/SUP], Michele Gastaldelli[SUP] 3 [/SUP], Annalisa Salviato[SUP] 3 [/SUP], Francesco Bonfante[SUP] 3 [/SUP], Sergei Kosakovsky Pond[SUP] 4 [/SUP], Sander Herfst[SUP] 5 [/SUP], Ron Fouchier[SUP] 5 [/SUP], Jochen Wilhelm[SUP] 6 [/SUP], Hans-Dieter Klenk[SUP] 1 [/SUP], Mikhail Matrosovich[SUP] 1 [/SUP]
Affiliations
Abstract
The hemagglutinin (HA) of A/H3N2 pandemic influenza viruses (IAVs) of 1968 differed from its inferred avian precursor by eight amino acid substitutions. To determine their phenotypic effects, we studied recombinant variants of A/Hong Kong/1/1968 virus containing either human-type or avian-type amino acids in the corresponding positions of HA. The precursor HA displayed receptor binding profile and high conformational stability typical for duck IAVs. Substitutions Q226L and G228S, in addition to their known effects on receptor specificity and replication, marginally decreased HA stability. Substitutions R62I, D63N, D81N and N193S reduced HA binding avidity. Substitutions R62I, D81N and A144G promoted viral replication in human airway epithelial cultures. Analysis of HA sequences revealed that substitutions D63N and D81N accompanied by the addition of N-glycans represent common markers of avian H3 HA adaptation to mammals. Our results advance understanding of genotypic and phenotypic changes in IAV HA required for avian-to-human adaptation and pandemic emergence.
. 2021 Sep 23;17(9):e1009566.
doi: 10.1371/journal.ppat.1009566. Online ahead of print.
Characterization of changes in the hemagglutinin that accompanied the emergence of H3N2/1968 pandemic influenza viruses
Johanna West[SUP] 1 [/SUP], Juliane Röder[SUP] 1 [/SUP], Tatyana Matrosovich[SUP] 1 [/SUP], Jana Beicht[SUP] 1 [/SUP], Jan Baumann[SUP] 1 [/SUP], Nancy Mounogou Kouassi[SUP] 1 [/SUP], Jennifer Doedt[SUP] 1 [/SUP], Nicolai Bovin[SUP] 2 [/SUP], Gianpiero Zamperin[SUP] 3 [/SUP], Michele Gastaldelli[SUP] 3 [/SUP], Annalisa Salviato[SUP] 3 [/SUP], Francesco Bonfante[SUP] 3 [/SUP], Sergei Kosakovsky Pond[SUP] 4 [/SUP], Sander Herfst[SUP] 5 [/SUP], Ron Fouchier[SUP] 5 [/SUP], Jochen Wilhelm[SUP] 6 [/SUP], Hans-Dieter Klenk[SUP] 1 [/SUP], Mikhail Matrosovich[SUP] 1 [/SUP]
Affiliations
- PMID: 34555124
- DOI: 10.1371/journal.ppat.1009566
Abstract
The hemagglutinin (HA) of A/H3N2 pandemic influenza viruses (IAVs) of 1968 differed from its inferred avian precursor by eight amino acid substitutions. To determine their phenotypic effects, we studied recombinant variants of A/Hong Kong/1/1968 virus containing either human-type or avian-type amino acids in the corresponding positions of HA. The precursor HA displayed receptor binding profile and high conformational stability typical for duck IAVs. Substitutions Q226L and G228S, in addition to their known effects on receptor specificity and replication, marginally decreased HA stability. Substitutions R62I, D63N, D81N and N193S reduced HA binding avidity. Substitutions R62I, D81N and A144G promoted viral replication in human airway epithelial cultures. Analysis of HA sequences revealed that substitutions D63N and D81N accompanied by the addition of N-glycans represent common markers of avian H3 HA adaptation to mammals. Our results advance understanding of genotypic and phenotypic changes in IAV HA required for avian-to-human adaptation and pandemic emergence.