tetano
Editor, Senior Moderator
PLoS One
. 2025 Oct 27;20(10):e0335070.
doi: 10.1371/journal.pone.0335070. eCollection 2025. Sequencing and analysis of 131 SARS-CoV-2 isolates in previously sampled and unsampled regions of Jordan from 2020 to 2023
Rame Khasawneh[SUP] 1 [/SUP], Andrew W Bartlow[SUP] 2 [/SUP], Shirin Almharat[SUP] 1 [/SUP], Abdullah Almuhasen[SUP] 1 [/SUP], Ali Alhuniti[SUP] 1 [/SUP], Lena Hajaya[SUP] 1 [/SUP], Al Anood Al Habashneh[SUP] 1 [/SUP], Osama Alshudefat[SUP] 1 [/SUP], Mohammad Dojan[SUP] 1 [/SUP], Abdelghani Tbakhi[SUP] 3 [/SUP], Zeena Shaheen[SUP] 4 [/SUP], Eiad Atwa[SUP] 4 [/SUP], Colin Ps Kruse[SUP] 2 [/SUP], Osama Alsmadi[SUP] 4 [/SUP], Samuel I Koehler[SUP] 2 [/SUP]
Affiliations
The Hashemite Kingdom of Jordan remains an understudied country for next generation sequencing analysis of SARS-CoV-2 genomes collected during the 2019 pandemic. Here we provide 131 additional reference genomes collected between 2020-2023 from SARS-CoV-2-positive patients across Jordan. Phylogenetic analysis supports existing pandemic narratives of changing clade dominance over time and adds genomes in novel Jordanian locations and timepoints to make Jordan SARS-CoV-2 databases more comprehensive. Samples from the less-sequenced cities of Ajloun, Jaresh, Karak, and Madaba identified previously unreported lineages while Amman, Irbid, and Zarqa have existing sequencing efforts bolstered. Despite many incomplete patient records and a relatively small sample size, we observe interesting symptom patterns that support existing global and Jordanian pandemic narratives. We note how in-country COVID-19 pandemic genomic studies showcase Jordan's efforts to expand next generation sequencing capabilities, especially through the leveraging of EDGE COVID-19, a bioinformatics platform for performing rapid, batched analysis of SARS-CoV-2 sequencing that streamlines sample processing prepared from a network of hospital locations.
. 2025 Oct 27;20(10):e0335070.
doi: 10.1371/journal.pone.0335070. eCollection 2025. Sequencing and analysis of 131 SARS-CoV-2 isolates in previously sampled and unsampled regions of Jordan from 2020 to 2023
Rame Khasawneh[SUP] 1 [/SUP], Andrew W Bartlow[SUP] 2 [/SUP], Shirin Almharat[SUP] 1 [/SUP], Abdullah Almuhasen[SUP] 1 [/SUP], Ali Alhuniti[SUP] 1 [/SUP], Lena Hajaya[SUP] 1 [/SUP], Al Anood Al Habashneh[SUP] 1 [/SUP], Osama Alshudefat[SUP] 1 [/SUP], Mohammad Dojan[SUP] 1 [/SUP], Abdelghani Tbakhi[SUP] 3 [/SUP], Zeena Shaheen[SUP] 4 [/SUP], Eiad Atwa[SUP] 4 [/SUP], Colin Ps Kruse[SUP] 2 [/SUP], Osama Alsmadi[SUP] 4 [/SUP], Samuel I Koehler[SUP] 2 [/SUP]
Affiliations
- PMID: 41144542
- DOI: 10.1371/journal.pone.0335070
The Hashemite Kingdom of Jordan remains an understudied country for next generation sequencing analysis of SARS-CoV-2 genomes collected during the 2019 pandemic. Here we provide 131 additional reference genomes collected between 2020-2023 from SARS-CoV-2-positive patients across Jordan. Phylogenetic analysis supports existing pandemic narratives of changing clade dominance over time and adds genomes in novel Jordanian locations and timepoints to make Jordan SARS-CoV-2 databases more comprehensive. Samples from the less-sequenced cities of Ajloun, Jaresh, Karak, and Madaba identified previously unreported lineages while Amman, Irbid, and Zarqa have existing sequencing efforts bolstered. Despite many incomplete patient records and a relatively small sample size, we observe interesting symptom patterns that support existing global and Jordanian pandemic narratives. We note how in-country COVID-19 pandemic genomic studies showcase Jordan's efforts to expand next generation sequencing capabilities, especially through the leveraging of EDGE COVID-19, a bioinformatics platform for performing rapid, batched analysis of SARS-CoV-2 sequencing that streamlines sample processing prepared from a network of hospital locations.