Giuseppe
Emeritus
[Source: PLoS ONE, full page: (LINK). Abstract, edited.]
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Residual Baculovirus in Insect Cell-Derived Influenza Virus-Like Particle Preparations Enhances Immunogenicity
Irina Margine<SUP>1</SUP><SUP>,</SUP><SUP>2</SUP>, Luis Martinez-Gil<SUP>1</SUP>, Yi-ying Chou<SUP>1</SUP><SUP>,</SUP><SUP>2</SUP>, Florian Krammer<SUP>1</SUP><SUP>*</SUP>
<SUP></SUP>
1 Department of Microbiology, Mount Sinai School of Medicine, New York, New York, United States of America, 2 Graduate School of Biological Sciences, Mount Sinai School of Medicine, New York, New York, United States of America
Abstract
Influenza virus-like particles are currently evaluated in clinical trials as vaccine candidates for influenza viruses. Most commonly they are produced in baculovirus- or mammalian- expression systems. Here we used different vaccination schemes in order to systematically compare virus-like particle preparations generated in the two systems. Our work shows significant differences in immunogenicity between the two, and indicates superior and broader immune responses induced by the baculovirus-derived constructs. We demonstrate that these differences critically influence protection and survival in a mouse model of influenza virus infection. Finally, we show that the enhanced immunogenicity of the baculovirus-derived virus-like particles is caused by contamination with residual baculovirus which activates the innate immune response at the site of inoculation.
Citation: Margine I, Martinez-Gil L, Chou Y-y, Krammer F (2012) Residual Baculovirus in Insect Cell-Derived Influenza Virus-Like Particle Preparations Enhances Immunogenicity. PLoS ONE 7(12): e51559. doi:10.1371/journal.pone.0051559
Editor: Paul Zhou, Pasteur Institute of Shanghai, Chinese Academy of Science, China
Received: August 20, 2012; Accepted: November 5, 2012; Published: December 7, 2012
Copyright: ? 2012 Margine et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was partially supported by PATH and CEIRS (Centers for Excellence for Influenza Research and Surveillance, HHSN26620070010C) grants to Dr. Peter Palese. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
* E-mail: florian.krammer@mssm.edu
-Irina Margine<SUP>1</SUP><SUP>,</SUP><SUP>2</SUP>, Luis Martinez-Gil<SUP>1</SUP>, Yi-ying Chou<SUP>1</SUP><SUP>,</SUP><SUP>2</SUP>, Florian Krammer<SUP>1</SUP><SUP>*</SUP>
<SUP></SUP>
1 Department of Microbiology, Mount Sinai School of Medicine, New York, New York, United States of America, 2 Graduate School of Biological Sciences, Mount Sinai School of Medicine, New York, New York, United States of America
Abstract
Influenza virus-like particles are currently evaluated in clinical trials as vaccine candidates for influenza viruses. Most commonly they are produced in baculovirus- or mammalian- expression systems. Here we used different vaccination schemes in order to systematically compare virus-like particle preparations generated in the two systems. Our work shows significant differences in immunogenicity between the two, and indicates superior and broader immune responses induced by the baculovirus-derived constructs. We demonstrate that these differences critically influence protection and survival in a mouse model of influenza virus infection. Finally, we show that the enhanced immunogenicity of the baculovirus-derived virus-like particles is caused by contamination with residual baculovirus which activates the innate immune response at the site of inoculation.
Citation: Margine I, Martinez-Gil L, Chou Y-y, Krammer F (2012) Residual Baculovirus in Insect Cell-Derived Influenza Virus-Like Particle Preparations Enhances Immunogenicity. PLoS ONE 7(12): e51559. doi:10.1371/journal.pone.0051559
Editor: Paul Zhou, Pasteur Institute of Shanghai, Chinese Academy of Science, China
Received: August 20, 2012; Accepted: November 5, 2012; Published: December 7, 2012
Copyright: ? 2012 Margine et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was partially supported by PATH and CEIRS (Centers for Excellence for Influenza Research and Surveillance, HHSN26620070010C) grants to Dr. Peter Palese. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
* E-mail: florian.krammer@mssm.edu
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