tetano
Editor, Senior Moderator
PLoS One
. 2021 Jan 15;16(1):e0244885.
doi: 10.1371/journal.pone.0244885. eCollection 2021.
A novel aqueous extract from rice fermented with Aspergillus oryzae and Saccharomyces cerevisiae possesses an anti-influenza A virus activity
Masaki Shoji[SUP] 1 [/SUP], Minami Sugimoto[SUP] 1 [/SUP], Kosuke Matsuno[SUP] 2 [/SUP], Yoko Fujita[SUP] 3 4 [/SUP], Tomohiro Mii[SUP] 2 [/SUP], Satomi Ayaki[SUP] 2 [/SUP], Misa Takeuchi[SUP] 1 [/SUP], Saki Yamaji[SUP] 1 [/SUP], Narue Tanaka[SUP] 1 [/SUP], Etsuhisa Takahashi[SUP] 5 [/SUP], Takeshi Noda[SUP] 3 4 [/SUP], Hiroshi Kido[SUP] 5 [/SUP], Takaaki Tokuyama[SUP] 2 [/SUP], Takahito Tokuyama[SUP] 2 [/SUP], Takashi Tokuyama[SUP] 2 [/SUP], Takashi Kuzuhara[SUP] 1 [/SUP]
Affiliations
Abstract
Human influenza virus infections occur annually worldwide and are associated with high morbidity and mortality. Hence, development of novel anti-influenza drugs is urgently required. Rice Power? extract developed by the Yushin Brewer Co. Ltd. is a novel aqueous extract of rice obtained via saccharization and fermentation with various microorganisms, such as Aspergillus oryzae, yeast [such as Saccharomyces cerevisiae], and lactic acid bacteria, possessing various biological and pharmacological properties. In our previous experimental screening with thirty types of Rice Power? extracts, we observed that the 30th Rice Power? (Y30) extract promoted the survival of influenza A virus-infected Madin-Darby canine kidney (MDCK) cells. Therefore, to identify compounds for the development of novel anti-influenza drugs, we aimed to investigate whether the Y30 extract exhibits anti-influenza A virus activity. In the present study, we demonstrated that the Y30 extract strongly promoted the survival of influenza A H1N1 Puerto Rico 8/34 (A/PR/8/34), California 7/09, or H3N2 Aichi 2/68 (A/Aichi/2/68) viruses-infected MDCK cells and inhibited A/PR/8/34 or A/Aichi/2/68 viruses infection and growth in the co-treatment and pre-infection experiments. The pre-treatment of Y30 extract on MDCK cells did not induce anti-influenza activity in the cell. The Y30 extract did not significantly affect influenza A virus hemagglutination, and neuraminidase and RNA-dependent RNA polymerase activities. Interestingly, the electron microscopy experiment revealed that the Y30 extract disrupts the integrity of influenza A virus particles by permeabilizing the viral membrane envelope, suggesting that Y30 extract has a direct virucidal effect against influenza A virus. Furthermore, we observed that compared to the ethyl acetate (EtOAc) extract, the water extract of Y30 extract considerably promoted the survival of cells infected with A/PR/8/34 virus. These results indicated that more anti-influenza components were present in the water extract of Y30 extract than in the EtOAc extract. Our results highlight the potential of a rice extract fermented with A. oryzae and S. cerevisiae as an anti-influenza medicine and a drug source for the development of anti-influenza compounds.
. 2021 Jan 15;16(1):e0244885.
doi: 10.1371/journal.pone.0244885. eCollection 2021.
A novel aqueous extract from rice fermented with Aspergillus oryzae and Saccharomyces cerevisiae possesses an anti-influenza A virus activity
Masaki Shoji[SUP] 1 [/SUP], Minami Sugimoto[SUP] 1 [/SUP], Kosuke Matsuno[SUP] 2 [/SUP], Yoko Fujita[SUP] 3 4 [/SUP], Tomohiro Mii[SUP] 2 [/SUP], Satomi Ayaki[SUP] 2 [/SUP], Misa Takeuchi[SUP] 1 [/SUP], Saki Yamaji[SUP] 1 [/SUP], Narue Tanaka[SUP] 1 [/SUP], Etsuhisa Takahashi[SUP] 5 [/SUP], Takeshi Noda[SUP] 3 4 [/SUP], Hiroshi Kido[SUP] 5 [/SUP], Takaaki Tokuyama[SUP] 2 [/SUP], Takahito Tokuyama[SUP] 2 [/SUP], Takashi Tokuyama[SUP] 2 [/SUP], Takashi Kuzuhara[SUP] 1 [/SUP]
Affiliations
- PMID: 33449947
- DOI: 10.1371/journal.pone.0244885
Abstract
Human influenza virus infections occur annually worldwide and are associated with high morbidity and mortality. Hence, development of novel anti-influenza drugs is urgently required. Rice Power? extract developed by the Yushin Brewer Co. Ltd. is a novel aqueous extract of rice obtained via saccharization and fermentation with various microorganisms, such as Aspergillus oryzae, yeast [such as Saccharomyces cerevisiae], and lactic acid bacteria, possessing various biological and pharmacological properties. In our previous experimental screening with thirty types of Rice Power? extracts, we observed that the 30th Rice Power? (Y30) extract promoted the survival of influenza A virus-infected Madin-Darby canine kidney (MDCK) cells. Therefore, to identify compounds for the development of novel anti-influenza drugs, we aimed to investigate whether the Y30 extract exhibits anti-influenza A virus activity. In the present study, we demonstrated that the Y30 extract strongly promoted the survival of influenza A H1N1 Puerto Rico 8/34 (A/PR/8/34), California 7/09, or H3N2 Aichi 2/68 (A/Aichi/2/68) viruses-infected MDCK cells and inhibited A/PR/8/34 or A/Aichi/2/68 viruses infection and growth in the co-treatment and pre-infection experiments. The pre-treatment of Y30 extract on MDCK cells did not induce anti-influenza activity in the cell. The Y30 extract did not significantly affect influenza A virus hemagglutination, and neuraminidase and RNA-dependent RNA polymerase activities. Interestingly, the electron microscopy experiment revealed that the Y30 extract disrupts the integrity of influenza A virus particles by permeabilizing the viral membrane envelope, suggesting that Y30 extract has a direct virucidal effect against influenza A virus. Furthermore, we observed that compared to the ethyl acetate (EtOAc) extract, the water extract of Y30 extract considerably promoted the survival of cells infected with A/PR/8/34 virus. These results indicated that more anti-influenza components were present in the water extract of Y30 extract than in the EtOAc extract. Our results highlight the potential of a rice extract fermented with A. oryzae and S. cerevisiae as an anti-influenza medicine and a drug source for the development of anti-influenza compounds.