tetano
Editor, Senior Moderator
Proteomics. 2014 May 28. doi: 10.1002/pmic.201400030. [Epub ahead of print]
Plasma proteomic analysis of patients infected with H1N1 influenza virus.
Choi S1, Lim JY, Kim Y, Song M, Jung WW, Seo JB, Lee J, Sul D.
Author information
Abstract
This study profiled the plasma proteins of patients infected by the 2011 H1N1 influenza virus. Differential protein expression was identified in plasma obtained from non-infected control subjects (n = 15) and H1N1 infected subjects (n = 15). Plasma proteins were separated by a two-dimensional electrophoresis large gel system and identified by nano-ultra performance liquid chromatography-mass spectrometry. Western blot assays were performed to validate proteins. Eight plasma proteins were up-regulated and six proteins were down-regulated among 3316 plasma proteins in the H1N1 infected group as compared with the control group. Of 14 up-and down-regulated proteins, nine plasma proteins were validated by Western blot analysis. Putative protein FAM 157A, leucine rich alpha 2 glycoprotein, serum amyloid A protein, and dual oxidase 1 showed significant differential expression. The identified plasma proteins could be potential candidates for biomarkers of H1N1 influenza viral infection. Further studies are needed to develop these proteins as diagnostic biomarkers. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
H1N1; biomarkers; influenza virus; plasma proteins
PMID:
24888898
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24888898
Plasma proteomic analysis of patients infected with H1N1 influenza virus.
Choi S1, Lim JY, Kim Y, Song M, Jung WW, Seo JB, Lee J, Sul D.
Author information
Abstract
This study profiled the plasma proteins of patients infected by the 2011 H1N1 influenza virus. Differential protein expression was identified in plasma obtained from non-infected control subjects (n = 15) and H1N1 infected subjects (n = 15). Plasma proteins were separated by a two-dimensional electrophoresis large gel system and identified by nano-ultra performance liquid chromatography-mass spectrometry. Western blot assays were performed to validate proteins. Eight plasma proteins were up-regulated and six proteins were down-regulated among 3316 plasma proteins in the H1N1 infected group as compared with the control group. Of 14 up-and down-regulated proteins, nine plasma proteins were validated by Western blot analysis. Putative protein FAM 157A, leucine rich alpha 2 glycoprotein, serum amyloid A protein, and dual oxidase 1 showed significant differential expression. The identified plasma proteins could be potential candidates for biomarkers of H1N1 influenza viral infection. Further studies are needed to develop these proteins as diagnostic biomarkers. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
H1N1; biomarkers; influenza virus; plasma proteins
PMID:
24888898
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24888898