tetano
Editor, Senior Moderator
J Vet Res. 2017 Sep 19;61(3):259-265. doi: 10.1515/jvetres-2017-0036. eCollection 2017 Sep.
[h=1]Pig Lung Immune Cytokine Response to the Swine Influenza Virus and the Actinobacillus Pleuropneumoniae Infection.[/h] Czyżewska-Dors E[SUP]1[/SUP], Dors A[SUP]1[/SUP], Kwit K[SUP]1[/SUP], Stasiak E[SUP]1[/SUP], Pomorska-M?l M[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]Introduction:[/h] The aim of this study was to evaluate and compare the local innate immune response to the swine influenza virus (SIV) and Actinobacillus pleuropneumoniae (App) infection in pigs.
[h=4]Material and Methods:[/h] The study was performed on 37 seven-week-old pigs, divided into four groups: App-infected (n=11), App+SIV-infected (n=11), SIV-infected (n=11), and control (n=4). Lung samples were collected, following euthanasia, on the 2[SUP]nd[/SUP] and 4[SUP]th[/SUP] dpi (three piglets per inoculated group) and on the 10[SUP]th[/SUP] dpi (remaining inoculated and control pigs). Lung concentrations of IL-1β, IL-6, IL-8, TNF-α, IL-10, IFN-α, and IFN-γ were analysed with the use of commercial porcine cytokine ELISA kits.
[h=4]Results:[/h] Lung concentrations of IL-1β, IL-6, IL-8, TNF-α, IFN-α, and IFN-γ were induced in SIV-infected and App+SIV-infected pigs. In the lung tissue of App-infected pigs, only concentrations of IL-1β, IL-6, IL-8, and IFN-γ were elevated. Additionally, in App+SIV-infected pigs, significantly greater concentrations of IL-1β, IL-8, and IFN-α were found when compared with pigs infected with either SIV or App alone. In each tested group, the lung concentration of IL-10 remained unchanged during the entire study.
[h=4]Conclusion:[/h] The results of the study indicate that the experimental infection of pigs with SIV or App alone and co-infection with both pathogens induced a local lung inflammatory response. However, the local cytokine response was considerably higher in co-infected pigs compared to single-infected pigs.
[h=4]KEYWORDS:[/h] Actinobacillus pleuropneumoniae; cytokines; local immunity; pigs; swine influenza virus
PMID: 29978082 PMCID: PMC5894434 DOI: 10.1515/jvetres-2017-0036
Free PMC Article
[h=1]Pig Lung Immune Cytokine Response to the Swine Influenza Virus and the Actinobacillus Pleuropneumoniae Infection.[/h] Czyżewska-Dors E[SUP]1[/SUP], Dors A[SUP]1[/SUP], Kwit K[SUP]1[/SUP], Stasiak E[SUP]1[/SUP], Pomorska-M?l M[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]Introduction:[/h] The aim of this study was to evaluate and compare the local innate immune response to the swine influenza virus (SIV) and Actinobacillus pleuropneumoniae (App) infection in pigs.
[h=4]Material and Methods:[/h] The study was performed on 37 seven-week-old pigs, divided into four groups: App-infected (n=11), App+SIV-infected (n=11), SIV-infected (n=11), and control (n=4). Lung samples were collected, following euthanasia, on the 2[SUP]nd[/SUP] and 4[SUP]th[/SUP] dpi (three piglets per inoculated group) and on the 10[SUP]th[/SUP] dpi (remaining inoculated and control pigs). Lung concentrations of IL-1β, IL-6, IL-8, TNF-α, IL-10, IFN-α, and IFN-γ were analysed with the use of commercial porcine cytokine ELISA kits.
[h=4]Results:[/h] Lung concentrations of IL-1β, IL-6, IL-8, TNF-α, IFN-α, and IFN-γ were induced in SIV-infected and App+SIV-infected pigs. In the lung tissue of App-infected pigs, only concentrations of IL-1β, IL-6, IL-8, and IFN-γ were elevated. Additionally, in App+SIV-infected pigs, significantly greater concentrations of IL-1β, IL-8, and IFN-α were found when compared with pigs infected with either SIV or App alone. In each tested group, the lung concentration of IL-10 remained unchanged during the entire study.
[h=4]Conclusion:[/h] The results of the study indicate that the experimental infection of pigs with SIV or App alone and co-infection with both pathogens induced a local lung inflammatory response. However, the local cytokine response was considerably higher in co-infected pigs compared to single-infected pigs.
[h=4]KEYWORDS:[/h] Actinobacillus pleuropneumoniae; cytokines; local immunity; pigs; swine influenza virus
PMID: 29978082 PMCID: PMC5894434 DOI: 10.1515/jvetres-2017-0036
Free PMC Article