tetano
Editor, Senior Moderator
Pharmacol Res. 2020 Mar 20:104761. doi: 10.1016/j.phrs.2020.104761. [Epub ahead of print]
Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2).
Runfeng L[SUP]1[/SUP], Yunlong H[SUP]2[/SUP], Jicheng H[SUP]3[/SUP], Weiqi P[SUP]1[/SUP], Qinhai M[SUP]1[/SUP], Yongxia S[SUP]3[/SUP], Chufang L[SUP]1[/SUP], Jin Z[SUP]1[/SUP], Zhenhua J[SUP]2[/SUP], Haiming J[SUP]1[/SUP], Kui Z[SUP]3[/SUP], Shuxiang H[SUP]3[/SUP], Jun D[SUP]3[/SUP], Xiaobo L[SUP]3[/SUP], Xiaotao H[SUP]4[/SUP], Lin W[SUP]4[/SUP], Nanshan Z[SUP]1[/SUP], Zifeng Y[SUP]5[/SUP].
Author information
Abstract
PURPOSE:
Lianhuaqingwen (LH) as traditional Chinese medicine (TCM) formula has been used to treat influenza and exerted broad-spectrum antiviral effects on a series of influenza viruses and immune regulatory effects [1]. The goal of this study is to demonstrate the antiviral activity of LH against the novel SARS-CoV-2 virus and its potential effect in regulating host immune response.
METHODS:
The antiviral activity of LH against SARS-CoV-2 was assessed in Vero E6 cells using CPE and plaque reduction assay. The effect of LH on virion morphology was visualized under transmission electron microscope. Pro-inflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays.
RESULTS:
LH significantly inhibited SARS-CoV-2 replication in Vero E6 cells and markedly reduced pro-inflammatory cytokines (TNF-α, IL-6, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Furthermore, LH treatment resulted in abnormal particle morphology of virion in cells.
CONCLUSIONS:
LH significantly inhibits the SARS-COV-2 replication, affects virus morphology and exerts anti-inflammatory activity in vitro. These findings indicate that LH protects against the virus attack, making its use a novel strategy for controlling the COVID-19 disease.
Copyright ? 2020. Published by Elsevier Ltd.
KEYWORDS:
Lianhuaqingwen; SARS-CoV-2; anti-inflammatory; coronavirus
PMID:32205232DOI:10.1016/j.phrs.2020.104761
Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2).
Runfeng L[SUP]1[/SUP], Yunlong H[SUP]2[/SUP], Jicheng H[SUP]3[/SUP], Weiqi P[SUP]1[/SUP], Qinhai M[SUP]1[/SUP], Yongxia S[SUP]3[/SUP], Chufang L[SUP]1[/SUP], Jin Z[SUP]1[/SUP], Zhenhua J[SUP]2[/SUP], Haiming J[SUP]1[/SUP], Kui Z[SUP]3[/SUP], Shuxiang H[SUP]3[/SUP], Jun D[SUP]3[/SUP], Xiaobo L[SUP]3[/SUP], Xiaotao H[SUP]4[/SUP], Lin W[SUP]4[/SUP], Nanshan Z[SUP]1[/SUP], Zifeng Y[SUP]5[/SUP].
Author information
Abstract
PURPOSE:
Lianhuaqingwen (LH) as traditional Chinese medicine (TCM) formula has been used to treat influenza and exerted broad-spectrum antiviral effects on a series of influenza viruses and immune regulatory effects [1]. The goal of this study is to demonstrate the antiviral activity of LH against the novel SARS-CoV-2 virus and its potential effect in regulating host immune response.
METHODS:
The antiviral activity of LH against SARS-CoV-2 was assessed in Vero E6 cells using CPE and plaque reduction assay. The effect of LH on virion morphology was visualized under transmission electron microscope. Pro-inflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays.
RESULTS:
LH significantly inhibited SARS-CoV-2 replication in Vero E6 cells and markedly reduced pro-inflammatory cytokines (TNF-α, IL-6, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Furthermore, LH treatment resulted in abnormal particle morphology of virion in cells.
CONCLUSIONS:
LH significantly inhibits the SARS-COV-2 replication, affects virus morphology and exerts anti-inflammatory activity in vitro. These findings indicate that LH protects against the virus attack, making its use a novel strategy for controlling the COVID-19 disease.
Copyright ? 2020. Published by Elsevier Ltd.
KEYWORDS:
Lianhuaqingwen; SARS-CoV-2; anti-inflammatory; coronavirus
PMID:32205232DOI:10.1016/j.phrs.2020.104761