tetano
Editor, Senior Moderator
Appl Environ Microbiol. 2013 Jan 18. [Epub ahead of print]
Persistence of the 2009 Pandemic Influenza A (H1N1) Virus (pH1N1) on N95 Respirators.
Coulliette AD, Perry KA, Edwards J, Noble-Wang J.
Source
National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control, Atlanta, GA 30307.
Abstract
In the U.S., the 2009 pandemic influenza A (H1N1) virus (pH1N1) infected almost 20% of the population and caused >200,000 hospitalizations and >10,000 deaths during April 2009 to April 2010. On April 24, 2009, CDC posted interim guidance on infection control measures in healthcare settings specifically for pH1N1, which recommended filtering face respirators (FFRs) when in close contact with a suspected/confirmed infected individual and particularly when performing aerosol procedures. The persistence and infectivity of pH1N1 was evaluated on FFRs, specifically N95 respirators, under varying conditions of absolute humidity (AH; 4.1 millibar (mb), 6.5 mb, and 14.6 mb), sample matrices (2% fetal bovine serum (FBS), 5 mg/ml mucin, and viral media), and time (4, 12, 24, 48, 72, and 144 hours). pH1N1 was distributed onto N95 coupons (3.8 to 4.2 cm(2)), extracted by a vortex/centrifugation/filtration process, and the ability of remaining virus to replicate was quantified by ELISA to determine the log(10) concentration of infectious virus per coupon. Overall, pH1N1 remained infectious for 6 days with approximately 1 log(10) loss of viral concentrations over this time period. Time and AH both affected viral survival. We found significantly higher (p ≤0.01) reductions in virus concentrations at time-points beyond 24 to 72 hours (-0.52 log(10) reduction) and 144 hours (-0.74) at AHs of 6.5 mb (-0.53) and 14.6 mb (-0.47). This research supports the discarding of respirators after close contact with a person with suspected or confirmed influenza infection due to the virus's demonstrated ability to persist and remain infectious.
PMID:
23335770
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23335770
Persistence of the 2009 Pandemic Influenza A (H1N1) Virus (pH1N1) on N95 Respirators.
Coulliette AD, Perry KA, Edwards J, Noble-Wang J.
Source
National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control, Atlanta, GA 30307.
Abstract
In the U.S., the 2009 pandemic influenza A (H1N1) virus (pH1N1) infected almost 20% of the population and caused >200,000 hospitalizations and >10,000 deaths during April 2009 to April 2010. On April 24, 2009, CDC posted interim guidance on infection control measures in healthcare settings specifically for pH1N1, which recommended filtering face respirators (FFRs) when in close contact with a suspected/confirmed infected individual and particularly when performing aerosol procedures. The persistence and infectivity of pH1N1 was evaluated on FFRs, specifically N95 respirators, under varying conditions of absolute humidity (AH; 4.1 millibar (mb), 6.5 mb, and 14.6 mb), sample matrices (2% fetal bovine serum (FBS), 5 mg/ml mucin, and viral media), and time (4, 12, 24, 48, 72, and 144 hours). pH1N1 was distributed onto N95 coupons (3.8 to 4.2 cm(2)), extracted by a vortex/centrifugation/filtration process, and the ability of remaining virus to replicate was quantified by ELISA to determine the log(10) concentration of infectious virus per coupon. Overall, pH1N1 remained infectious for 6 days with approximately 1 log(10) loss of viral concentrations over this time period. Time and AH both affected viral survival. We found significantly higher (p ≤0.01) reductions in virus concentrations at time-points beyond 24 to 72 hours (-0.52 log(10) reduction) and 144 hours (-0.74) at AHs of 6.5 mb (-0.53) and 14.6 mb (-0.47). This research supports the discarding of respirators after close contact with a person with suspected or confirmed influenza infection due to the virus's demonstrated ability to persist and remain infectious.
PMID:
23335770
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23335770