tetano
Editor, Senior Moderator
J Med Virol. 2012 Nov;84(11):1699-702. doi: 10.1002/jmv.23374.
Performance of five FDA-approved rapid antigen tests in the detection of 2009 H1N1 influenza A virus.
Sutter DE, Worthy SA, Hensley DM, Maranich AM, Dolan DM, Fischer GW, Daum LT.
Source
Department of Pediatrics, San Antonio Military Medical Center, Ft Sam Houston, Texas. deena.sutter@amedd.army.mil.
Abstract
Rapid antigen tests are commonly used by clinicians for rapid, simple, point-of-care testing. Five rapid antigen tests were shown to have low sensitivity (40.3-58.8%) when compared to real-time RT-PCR using nasal wash specimens from patients with influenza-like-illness (N = 167) that were collected previously and confirmed as 2009 pandemic influenza A (H1N1)-positive by PCR. Rapid antigen test sensitivity correlated with virus levels in nasal secretions when comparisons were made to cycle threshold (C(T) ) values obtained from real-time RT-PCR. When C(T) values are <25 (equating to viral concentrations of >10(4) TCID(50) /ml) sensitivity for all five rapid antigen kits was high (range: 83-94% positive); however, when C(T) values are >30 (10(2) TCID(50) /ml), sensitivities of only 16-18% were observed for four of five rapid antigen kits. The Directigen EZ Flu A + B test detected more positive samples (35%) at lower viral concentrations with C(T) values >30 when compared with other commercial kits (P = 0.05). Rapid antigen test results must be interpreted with caution, and negative specimens may need confirmation by sensitive molecular assays. J. Med. Virol. 84:1699-1702, 2012. ? 2012 Wiley Periodicals, Inc.
Copyright ? 2012 Wiley Periodicals, Inc.
PMID:
22997071
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/22997071
Performance of five FDA-approved rapid antigen tests in the detection of 2009 H1N1 influenza A virus.
Sutter DE, Worthy SA, Hensley DM, Maranich AM, Dolan DM, Fischer GW, Daum LT.
Source
Department of Pediatrics, San Antonio Military Medical Center, Ft Sam Houston, Texas. deena.sutter@amedd.army.mil.
Abstract
Rapid antigen tests are commonly used by clinicians for rapid, simple, point-of-care testing. Five rapid antigen tests were shown to have low sensitivity (40.3-58.8%) when compared to real-time RT-PCR using nasal wash specimens from patients with influenza-like-illness (N = 167) that were collected previously and confirmed as 2009 pandemic influenza A (H1N1)-positive by PCR. Rapid antigen test sensitivity correlated with virus levels in nasal secretions when comparisons were made to cycle threshold (C(T) ) values obtained from real-time RT-PCR. When C(T) values are <25 (equating to viral concentrations of >10(4) TCID(50) /ml) sensitivity for all five rapid antigen kits was high (range: 83-94% positive); however, when C(T) values are >30 (10(2) TCID(50) /ml), sensitivities of only 16-18% were observed for four of five rapid antigen kits. The Directigen EZ Flu A + B test detected more positive samples (35%) at lower viral concentrations with C(T) values >30 when compared with other commercial kits (P = 0.05). Rapid antigen test results must be interpreted with caution, and negative specimens may need confirmation by sensitive molecular assays. J. Med. Virol. 84:1699-1702, 2012. ? 2012 Wiley Periodicals, Inc.
Copyright ? 2012 Wiley Periodicals, Inc.
PMID:
22997071
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/22997071