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Vaccine
Article in Press, Uncorrected Proof - Note to users
doi:10.1016/j.vaccine.2010.02.088 | How to Cite or Link Using DOI
Copyright ? 2010 Elsevier Ltd All rights reserved.
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Peak frequencies of circulating human influenza-specific antibody secreting cells correlate with serum antibody response after immunization
References and further reading may be available for this article. To view references and further reading you must purchase this article.
Jessica L. Hallileya, Shuya Kyua, James J. Kobieb, Edward E. Walshc, Ann R. Falseyc, Troy D. Randallb, John Treanorc, Changyong Fengd, Ignacio Sanzb and F. Eun-Hyung Leea, Corresponding Author Contact Information, E-mail The Corresponding Author
a Division of Pulmonary & Critical Care Medicine, University of Rochester Medical Center, United States
b Division of Allergy, Immunology and Rheumatology, University of Rochester Medical Center, United States
c Division of Infectious Diseases, University of Rochester Medical Center, United States
d Department of Biostatistics and Computational Biology, University of Rochester Medical Center, United States
Received 3 November 2009;
revised 22 January 2010;
accepted 17 February 2010.
Available online 16 March 2010.
Abstract
Upon vaccination, B cells differentiate into antibody secreting cells (ASCs) that migrate via the circulation to tissues. The kinetics of this response and the relationship of circulating ASCs to protective antibody titers have not been completely explored.
Methods
Influenza-specific and total-IgG ASCs were enumerated by Elispot and flow cytometry daily in the blood in 6 healthy adults after trivalent influenza vaccination (TIV).
Results
Peak H1-specific IgG ASC frequencies occurred variably from day 5 to 8 and correlated with the fold-rise rise in hemagglutination inhibition (HAI titers); r = 0.91, p = 0.006. H3-specific IgG ASC frequencies correlated less well, perhaps due to a mismatch of the H3 protein in the vaccine and that used in the Elispot assay. Peak frequencies of vaccine-specific and total-IgG ASCs were 0.3% and 0.8%, respectively, of peripheral blood mononuclear cells (PBMC). Peak TIV-, H1-, H3-, and total-IgG ASC frequencies were 1736 ? 1133, 626 ? 520, 592 ? 463, and 4091 ? 2019 spots/106 PBMC, respectively. Peak TIV-, H1-, and H3-specific IgG ASC of total-IgG ASC frequencies constituted 63% ? 21, 26% ? 10, 22% ? 17, respectively.
Conclusion
After immunization with inactivated influenza vaccine the peak in influenza-specific ASC frequencies is variable but correlates well with the magnitude of protective HAI responses.
http://www.sciencedirect.com/scienc...serid=10&md5=baab7b816b4d85f365eae9978a35b123
Article in Press, Uncorrected Proof - Note to users
doi:10.1016/j.vaccine.2010.02.088 | How to Cite or Link Using DOI
Copyright ? 2010 Elsevier Ltd All rights reserved.
Permissions & Reprints
Peak frequencies of circulating human influenza-specific antibody secreting cells correlate with serum antibody response after immunization
References and further reading may be available for this article. To view references and further reading you must purchase this article.
Jessica L. Hallileya, Shuya Kyua, James J. Kobieb, Edward E. Walshc, Ann R. Falseyc, Troy D. Randallb, John Treanorc, Changyong Fengd, Ignacio Sanzb and F. Eun-Hyung Leea, Corresponding Author Contact Information, E-mail The Corresponding Author
a Division of Pulmonary & Critical Care Medicine, University of Rochester Medical Center, United States
b Division of Allergy, Immunology and Rheumatology, University of Rochester Medical Center, United States
c Division of Infectious Diseases, University of Rochester Medical Center, United States
d Department of Biostatistics and Computational Biology, University of Rochester Medical Center, United States
Received 3 November 2009;
revised 22 January 2010;
accepted 17 February 2010.
Available online 16 March 2010.
Abstract
Upon vaccination, B cells differentiate into antibody secreting cells (ASCs) that migrate via the circulation to tissues. The kinetics of this response and the relationship of circulating ASCs to protective antibody titers have not been completely explored.
Methods
Influenza-specific and total-IgG ASCs were enumerated by Elispot and flow cytometry daily in the blood in 6 healthy adults after trivalent influenza vaccination (TIV).
Results
Peak H1-specific IgG ASC frequencies occurred variably from day 5 to 8 and correlated with the fold-rise rise in hemagglutination inhibition (HAI titers); r = 0.91, p = 0.006. H3-specific IgG ASC frequencies correlated less well, perhaps due to a mismatch of the H3 protein in the vaccine and that used in the Elispot assay. Peak frequencies of vaccine-specific and total-IgG ASCs were 0.3% and 0.8%, respectively, of peripheral blood mononuclear cells (PBMC). Peak TIV-, H1-, H3-, and total-IgG ASC frequencies were 1736 ? 1133, 626 ? 520, 592 ? 463, and 4091 ? 2019 spots/106 PBMC, respectively. Peak TIV-, H1-, and H3-specific IgG ASC of total-IgG ASC frequencies constituted 63% ? 21, 26% ? 10, 22% ? 17, respectively.
Conclusion
After immunization with inactivated influenza vaccine the peak in influenza-specific ASC frequencies is variable but correlates well with the magnitude of protective HAI responses.
http://www.sciencedirect.com/scienc...serid=10&md5=baab7b816b4d85f365eae9978a35b123
