tetano
Editor, Senior Moderator
Open Forum Infect Dis
. 2020 Oct 14;7(11)
faa488.
doi: 10.1093/ofid/ofaa488. eCollection 2020 Nov.
Evaluation of Nasopharyngeal Swab Collection Techniques for Nucleic Acid Recovery and Participant Experience: Recommendations for COVID-19 Diagnostics
Natalie N Kinloch[SUP] 1 2 [/SUP], Aniqa Shahid[SUP] 1 2 [/SUP], Gordon Ritchie[SUP] 3 4 [/SUP], Winnie Dong[SUP] 2 [/SUP], Tanya Lawson[SUP] 3 [/SUP], Julio S G Montaner[SUP] 2 5 [/SUP], Marc G Romney[SUP] 3 4 [/SUP], Aleksandra Stefanovic[SUP] 3 4 [/SUP], Nancy Matic[SUP] 3 4 [/SUP], Chanson J Brumme[SUP] 2 5 [/SUP], Christopher F Lowe[SUP] 3 4 [/SUP], Zabrina L Brumme[SUP] 1 2 [/SUP], Victor Leung[SUP] 3 4 5 [/SUP]
Affiliations
Abstract
Nasopharyngeal swabs are critical to the diagnosis of respiratory infections including coronavirus disease 2019, but collection techniques vary. We compared 2 recommended nasopharyngeal swab collection techniques in adult volunteers and found that swab rotation following nasopharyngeal contact did not recover additional nucleic acid (as measured by human DNA/RNA copy number). Rotation was also less tolerable for participants. Notably, both discomfort and nucleic acid recovery were significantly higher in Asian participants, consistent with nasal anatomy differences. Our results suggest that it is unnecessary to rotate the swab in place following contact with the nasopharynx and reveal that procedural discomfort levels can differ by ethnicity.
Keywords: COVID-19; RT-ddPCR; biological material; ddPCR; ethnicity; nasopharyngeal swab; participant experience; sample quality.
. 2020 Oct 14;7(11)
faa488.doi: 10.1093/ofid/ofaa488. eCollection 2020 Nov.
Evaluation of Nasopharyngeal Swab Collection Techniques for Nucleic Acid Recovery and Participant Experience: Recommendations for COVID-19 Diagnostics
Natalie N Kinloch[SUP] 1 2 [/SUP], Aniqa Shahid[SUP] 1 2 [/SUP], Gordon Ritchie[SUP] 3 4 [/SUP], Winnie Dong[SUP] 2 [/SUP], Tanya Lawson[SUP] 3 [/SUP], Julio S G Montaner[SUP] 2 5 [/SUP], Marc G Romney[SUP] 3 4 [/SUP], Aleksandra Stefanovic[SUP] 3 4 [/SUP], Nancy Matic[SUP] 3 4 [/SUP], Chanson J Brumme[SUP] 2 5 [/SUP], Christopher F Lowe[SUP] 3 4 [/SUP], Zabrina L Brumme[SUP] 1 2 [/SUP], Victor Leung[SUP] 3 4 5 [/SUP]
Affiliations
- PMID: 33235889
- PMCID: PMC7665666
- DOI: 10.1093/ofid/ofaa488
Abstract
Nasopharyngeal swabs are critical to the diagnosis of respiratory infections including coronavirus disease 2019, but collection techniques vary. We compared 2 recommended nasopharyngeal swab collection techniques in adult volunteers and found that swab rotation following nasopharyngeal contact did not recover additional nucleic acid (as measured by human DNA/RNA copy number). Rotation was also less tolerable for participants. Notably, both discomfort and nucleic acid recovery were significantly higher in Asian participants, consistent with nasal anatomy differences. Our results suggest that it is unnecessary to rotate the swab in place following contact with the nasopharynx and reveal that procedural discomfort levels can differ by ethnicity.
Keywords: COVID-19; RT-ddPCR; biological material; ddPCR; ethnicity; nasopharyngeal swab; participant experience; sample quality.