tetano
Editor, Senior Moderator
J Virol. 2012 Apr 4. [Epub ahead of print]
Oct4+ stem/progenitor swine lung epithelial cells are targets for influenza virus replication.
Khatri M, Goyal SM, Saif YM.
Source
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH.
Abstract
We isolated stem/progenitor epithelial cells from the lungs of 4-6 week old pigs. The epithelial progenitor colony cells were surrounded by mesenchymal stromal cells. The progenitor epithelial colony cells expressed stem cell markers such as octamer binding transcription factor 4 (Oct4) and stage-specific embryonic antigen 1 (SSEA-1); and epithelial markers pancytokeratin, cytokeratin-18 and occludin but not mesenchymal (CD44, CD29, CD90) and hematopoietic (CD45) markers. The colony cells had extensive self-renewal potential and had capacity to undergo differentiation to alveolar type I- and type II-like pneumocytes. Additionally, these cells expressed sialic acid receptors and supported active replication of influenza virus, which was accompanied with cell lysis. Lysis of progenitor epithelial cells by influenza virus may cause marked reduction in potential of progenitor cells for self-renewal and for their ability to differentiate into specialized cells of lung. These observations suggest the possible involvement of lung stem/progenitor cells in influenza virus infection.
PMID:
22491467
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22491467
Oct4+ stem/progenitor swine lung epithelial cells are targets for influenza virus replication.
Khatri M, Goyal SM, Saif YM.
Source
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH.
Abstract
We isolated stem/progenitor epithelial cells from the lungs of 4-6 week old pigs. The epithelial progenitor colony cells were surrounded by mesenchymal stromal cells. The progenitor epithelial colony cells expressed stem cell markers such as octamer binding transcription factor 4 (Oct4) and stage-specific embryonic antigen 1 (SSEA-1); and epithelial markers pancytokeratin, cytokeratin-18 and occludin but not mesenchymal (CD44, CD29, CD90) and hematopoietic (CD45) markers. The colony cells had extensive self-renewal potential and had capacity to undergo differentiation to alveolar type I- and type II-like pneumocytes. Additionally, these cells expressed sialic acid receptors and supported active replication of influenza virus, which was accompanied with cell lysis. Lysis of progenitor epithelial cells by influenza virus may cause marked reduction in potential of progenitor cells for self-renewal and for their ability to differentiate into specialized cells of lung. These observations suggest the possible involvement of lung stem/progenitor cells in influenza virus infection.
PMID:
22491467
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22491467