tetano
Editor, Senior Moderator
JVI Accepts, published online ahead of print on 17 August 2011
J. Virol. doi:10.1128/JVI.05352-11
Novel reassortment of Eurasian Avian-like and pandemic/2009 influenza viruses in swine: infectious potential to humans
Huachen Zhu1,2,3, Boping Zhou2, Xiaohui Fan4, Tommy T.Y. Lam3, Jia Wang1,3, Antony Chen5, Xinchun Chen2, Honglin Chen1,3, Robert G. Webster6, Richard Webby6, Joseph S.M. Peiris3, David K. Smith1,3,*, and Yi Guan1,3,*
1 International Institute of Infection and Immunity, Shantou University Medical College, Shantou, Guangdong, China
2 State Key Laboratory of Emerging Infectious Diseases (Shenzhen Branch), Shenzhen Third People's Hospital, Shenzhen, Guangdong, China
3 State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China
4 Department of Microbiology, Guangxi Medical University, Guangxi, China
5 Wuxi Sangosho Pet Park Co., Ltd, Wuxi, Jiangsu, China
6 Division of Virology, Department of Infectious Disease, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
* Corresponding authors. Mailing address: State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China. Phone: (852) 2819-9830. Fax: (852) 2817-1958. Email: yguan{at}hku.hk or dsmithhk{at}gmail.com
arrow
ABSTRACT
Pigs are considered to be intermediate hosts and "mixing vessels", facilitating the genesis of pandemic influenza viruses as demonstrated by the emergence of the 2009 H1N1 pandemic (pdm/09) virus. The prevalence and repeated introduction of the pdm/09 virus to pigs raises the possibility to generate novel swine influenza viruses with the potential to infect humans. To address this, an active influenza surveillance program was conducted on slaughtered pigs in abattoirs in Southern China. Over 50% of the pigs tested were found to be seropositive for one or more H1 influenza viruses, most commonly pdm/09-like viruses. Out of 36 virus isolates detected, one group of novel reassortants had Eurasian avian-like swine H1N1 surface genes and pdm/09 internal genes. Animal experiments showed that this virus transmitted effectively from pig to pig and from pig to ferret, and it could also replicate in ex-vivo human lung tissue. Immunization against the 2009 pandemic virus gave only partial protection to ferrets. The continuing prevalence of the pdm/09 virus in pigs could lead to the genesis of novel swine reassortant viruses with the potential to infect humans.
http://jvi.asm.org/cgi/content/short/JVI.05352-11v1?rss=1
J. Virol. doi:10.1128/JVI.05352-11
Novel reassortment of Eurasian Avian-like and pandemic/2009 influenza viruses in swine: infectious potential to humans
Huachen Zhu1,2,3, Boping Zhou2, Xiaohui Fan4, Tommy T.Y. Lam3, Jia Wang1,3, Antony Chen5, Xinchun Chen2, Honglin Chen1,3, Robert G. Webster6, Richard Webby6, Joseph S.M. Peiris3, David K. Smith1,3,*, and Yi Guan1,3,*
1 International Institute of Infection and Immunity, Shantou University Medical College, Shantou, Guangdong, China
2 State Key Laboratory of Emerging Infectious Diseases (Shenzhen Branch), Shenzhen Third People's Hospital, Shenzhen, Guangdong, China
3 State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China
4 Department of Microbiology, Guangxi Medical University, Guangxi, China
5 Wuxi Sangosho Pet Park Co., Ltd, Wuxi, Jiangsu, China
6 Division of Virology, Department of Infectious Disease, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
* Corresponding authors. Mailing address: State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China. Phone: (852) 2819-9830. Fax: (852) 2817-1958. Email: yguan{at}hku.hk or dsmithhk{at}gmail.com
arrow
ABSTRACT
Pigs are considered to be intermediate hosts and "mixing vessels", facilitating the genesis of pandemic influenza viruses as demonstrated by the emergence of the 2009 H1N1 pandemic (pdm/09) virus. The prevalence and repeated introduction of the pdm/09 virus to pigs raises the possibility to generate novel swine influenza viruses with the potential to infect humans. To address this, an active influenza surveillance program was conducted on slaughtered pigs in abattoirs in Southern China. Over 50% of the pigs tested were found to be seropositive for one or more H1 influenza viruses, most commonly pdm/09-like viruses. Out of 36 virus isolates detected, one group of novel reassortants had Eurasian avian-like swine H1N1 surface genes and pdm/09 internal genes. Animal experiments showed that this virus transmitted effectively from pig to pig and from pig to ferret, and it could also replicate in ex-vivo human lung tissue. Immunization against the 2009 pandemic virus gave only partial protection to ferrets. The continuing prevalence of the pdm/09 virus in pigs could lead to the genesis of novel swine reassortant viruses with the potential to infect humans.
http://jvi.asm.org/cgi/content/short/JVI.05352-11v1?rss=1