tetano
Editor, Senior Moderator
J Virol. 2015 Aug 5. pii: JVI.01430-15. [Epub ahead of print]
[h=1]Novel Bat Influenza Virus NS1 Proteins Bind Double-Stranded RNA and Antagonize Host Innate Immunity.[/h] Turkington HL[SUP]1[/SUP], Juozapaitis M[SUP]2[/SUP], Kerry PS[SUP]3[/SUP], Aydillo T[SUP]4[/SUP], Ayllon J[SUP]4[/SUP], Garc?a-Sastre A[SUP]5[/SUP], Schwemmle M[SUP]6[/SUP], Hale BG[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We demonstrate that novel bat HL17NL10 and HL18NL11 influenza virus NS1 proteins are effective interferon-antagonists, but do not block general host gene expression. Solving the RNA-binding domain structures revealed the canonical NS1 symmetrical homodimer, and RNA-binding required conserved basic residues in this domain. Interferon-antagonism was strictly dependent on RNA-binding, and chimeric bat influenza viruses expressing NS1s defective in this activity were highly attenuated in interferon-competent cells, but not in cells unable to establish antiviral immunity.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26246567 [PubMed - as supplied by publisher]
[h=1]Novel Bat Influenza Virus NS1 Proteins Bind Double-Stranded RNA and Antagonize Host Innate Immunity.[/h] Turkington HL[SUP]1[/SUP], Juozapaitis M[SUP]2[/SUP], Kerry PS[SUP]3[/SUP], Aydillo T[SUP]4[/SUP], Ayllon J[SUP]4[/SUP], Garc?a-Sastre A[SUP]5[/SUP], Schwemmle M[SUP]6[/SUP], Hale BG[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We demonstrate that novel bat HL17NL10 and HL18NL11 influenza virus NS1 proteins are effective interferon-antagonists, but do not block general host gene expression. Solving the RNA-binding domain structures revealed the canonical NS1 symmetrical homodimer, and RNA-binding required conserved basic residues in this domain. Interferon-antagonism was strictly dependent on RNA-binding, and chimeric bat influenza viruses expressing NS1s defective in this activity were highly attenuated in interferon-competent cells, but not in cells unable to establish antiviral immunity.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26246567 [PubMed - as supplied by publisher]