tetano
Editor, Senior Moderator
Bioconjug Chem. 2016 May 3. [Epub ahead of print]
[h=1]Neuraminidase resistant sialosides for the detection of influenza viruses.[/h] He Y, Yang Y, Iyer SS.
[h=3]Abstract[/h] We report the synthesis of influenza virus neuraminidase (NA) resistant sialosides that include different glycoside linkages (C-, S- and triazole). These unnatural sialosides were printed onto glass slides to generate a small focused microarray. We evaluated the binding affinity of multiple lectins and compared the stability of these sialosides with O-linked sialosides towards influenza virus neuraminidase and intact virus. We demonstrated the ability of these molecules to capture eight different strains of influenza virus at ambient temperature without the addition of NA inhibitors. The glycans capture extremely low clinically relevant concentrations of viruses and each strain gives rise to a specific "fingerprint" binding pattern, which could potentially be used in rapid diagnostic tests.
PMID: 27139196 [PubMed - as supplied by publisher]
[h=1]Neuraminidase resistant sialosides for the detection of influenza viruses.[/h] He Y, Yang Y, Iyer SS.
[h=3]Abstract[/h] We report the synthesis of influenza virus neuraminidase (NA) resistant sialosides that include different glycoside linkages (C-, S- and triazole). These unnatural sialosides were printed onto glass slides to generate a small focused microarray. We evaluated the binding affinity of multiple lectins and compared the stability of these sialosides with O-linked sialosides towards influenza virus neuraminidase and intact virus. We demonstrated the ability of these molecules to capture eight different strains of influenza virus at ambient temperature without the addition of NA inhibitors. The glycans capture extremely low clinically relevant concentrations of viruses and each strain gives rise to a specific "fingerprint" binding pattern, which could potentially be used in rapid diagnostic tests.
PMID: 27139196 [PubMed - as supplied by publisher]