tetano
Editor, Senior Moderator
J Infect Chemother. 2018 Jun 1. pii: S1341-321X(18)30129-6. doi: 10.1016/j.jiac.2018.05.003. [Epub ahead of print]
[h=1]Neuraminidase inhibitor susceptibility profile of human influenza viruses during the 2016-2017 influenza season in Mainland China.[/h] Huang W[SUP]1[/SUP], Cheng Y[SUP]1[/SUP], Li X[SUP]1[/SUP], Tan M[SUP]1[/SUP], Wei H[SUP]1[/SUP], Zhao X[SUP]1[/SUP], Xiao N[SUP]1[/SUP], Dong J[SUP]1[/SUP], Wang D[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] To understand the current situation of antiviral-resistance of influenza viruses to neuraminidase inhibitors (NAIs) in Mainland China, The antiviral-resistant surveillance data of the circulating influenza viruses in Mainland China during the 2016-2017 influenza season were analyzed. The total 3215 influenza viruses were studied to determine 50% inhibitory concentration (IC[SUB]50[/SUB]) for oseltamivir and zanamivir using a fluorescence-based assay. Approximately 0.3% (n = 10) of viruses showed either highly reduced inhibition (HRI) or reduced inhibition (RI) against at least one NAI. The most common neuraminidase (NA) amino acid substitution was H275Y in A (H1N1)pdm09 virus, which confers HRI by oseltamivir. Two A (H1N1)pdm09 viruses contained a new NA amino acid substitution respectively, S110F and D151E, which confers RI by oseltamivir or/and zanamivir. Two B/Victoria-lineage viruses harbored a new NA amino acid substitution respectively, H134Q and S246P, which confers RI by zanamivir. One B/Victoria-lineage virus contained dual amino acid substitution NA P124T and V422I, which confers HRI by zanamivir. One B/Yamagata-lineage virus was a reassortant virus that haemagglutinin (HA) from B/Yamagata-lineage virus and NA from B/Victoria-lineage virus, defined as B/Yamagata-lineage virus confers RI by oseltamivir, but as B/Victoria-lineage virus confers normal inhibition by oseltamivir. All new substitutions that have not been reported before, the correlation of these substitutions and observed changes in IC[SUB]50[/SUB] should be further assessed. During the 2016-2017 influenza season in Mainland China the majority tested viruses were susceptible to oseltamivir and zanamivir. Hence, NAIs remain the recommended antiviral for treatment and prophylaxis of influenza virus infections.
[h=4]KEYWORDS:[/h] 50% inhibitory concentration; Antiviral-resistant; Influenza virus; Oseltmaivir; Zanamivir
PMID: 29866491 DOI: 10.1016/j.jiac.2018.05.003
[h=1]Neuraminidase inhibitor susceptibility profile of human influenza viruses during the 2016-2017 influenza season in Mainland China.[/h] Huang W[SUP]1[/SUP], Cheng Y[SUP]1[/SUP], Li X[SUP]1[/SUP], Tan M[SUP]1[/SUP], Wei H[SUP]1[/SUP], Zhao X[SUP]1[/SUP], Xiao N[SUP]1[/SUP], Dong J[SUP]1[/SUP], Wang D[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] To understand the current situation of antiviral-resistance of influenza viruses to neuraminidase inhibitors (NAIs) in Mainland China, The antiviral-resistant surveillance data of the circulating influenza viruses in Mainland China during the 2016-2017 influenza season were analyzed. The total 3215 influenza viruses were studied to determine 50% inhibitory concentration (IC[SUB]50[/SUB]) for oseltamivir and zanamivir using a fluorescence-based assay. Approximately 0.3% (n = 10) of viruses showed either highly reduced inhibition (HRI) or reduced inhibition (RI) against at least one NAI. The most common neuraminidase (NA) amino acid substitution was H275Y in A (H1N1)pdm09 virus, which confers HRI by oseltamivir. Two A (H1N1)pdm09 viruses contained a new NA amino acid substitution respectively, S110F and D151E, which confers RI by oseltamivir or/and zanamivir. Two B/Victoria-lineage viruses harbored a new NA amino acid substitution respectively, H134Q and S246P, which confers RI by zanamivir. One B/Victoria-lineage virus contained dual amino acid substitution NA P124T and V422I, which confers HRI by zanamivir. One B/Yamagata-lineage virus was a reassortant virus that haemagglutinin (HA) from B/Yamagata-lineage virus and NA from B/Victoria-lineage virus, defined as B/Yamagata-lineage virus confers RI by oseltamivir, but as B/Victoria-lineage virus confers normal inhibition by oseltamivir. All new substitutions that have not been reported before, the correlation of these substitutions and observed changes in IC[SUB]50[/SUB] should be further assessed. During the 2016-2017 influenza season in Mainland China the majority tested viruses were susceptible to oseltamivir and zanamivir. Hence, NAIs remain the recommended antiviral for treatment and prophylaxis of influenza virus infections.
[h=4]KEYWORDS:[/h] 50% inhibitory concentration; Antiviral-resistant; Influenza virus; Oseltmaivir; Zanamivir
PMID: 29866491 DOI: 10.1016/j.jiac.2018.05.003