tetano
Editor, Senior Moderator
Nat Immunol
. 2020 Sep 4.
doi: 10.1038/s41590-020-0782-6. Online ahead of print.
Broad and strong memory CD4 [SUP]+[/SUP] and CD8 [SUP]+[/SUP] T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19
Yanchun Peng[SUP] 1 2 [/SUP], Alexander J Mentzer[SUP] 3 4 5 [/SUP], Guihai Liu[SUP] 2 4 6 [/SUP], Xuan Yao[SUP] 1 2 4 [/SUP], Zixi Yin[SUP] 1 2 [/SUP], Danning Dong[SUP] 2 4 7 [/SUP], Wanwisa Dejnirattisai[SUP] 4 [/SUP], Timothy Rostron[SUP] 8 [/SUP], Piyada Supasa[SUP] 4 [/SUP], Chang Liu[SUP] 2 4 [/SUP], C?sar L?pez-Camacho[SUP] 3 4 [/SUP], Jose Slon-Campos[SUP] 4 [/SUP], Yuguang Zhao[SUP] 4 [/SUP], David I Stuart[SUP] 2 3 4 9 [/SUP], Guido C Paesen[SUP] 3 [/SUP], Jonathan M Grimes[SUP] 3 4 9 [/SUP], Alfred A Antson[SUP] 10 [/SUP], Oliver W Bayfield[SUP] 10 [/SUP], Dorothy E D P Hawkins[SUP] 10 [/SUP], De-Sheng Ker[SUP] 10 [/SUP], Beibei Wang[SUP] 2 4 [/SUP], Lance Turtle[SUP] 11 12 [/SUP], Krishanthi Subramaniam[SUP] 12 [/SUP], Paul Thomson[SUP] 12 [/SUP], Ping Zhang[SUP] 4 [/SUP], Christina Dold[SUP] 13 14 [/SUP], Jeremy Ratcliff[SUP] 4 [/SUP], Peter Simmonds[SUP] 4 [/SUP], Thushan de Silva[SUP] 15 [/SUP], Paul Sopp[SUP] 8 [/SUP], Dannielle Wellington[SUP] 1 2 [/SUP], Ushani Rajapaksa[SUP] 2 4 [/SUP], Yi-Ling Chen[SUP] 1 [/SUP], Mariolina Salio[SUP] 1 [/SUP], Giorgio Napolitani[SUP] 1 [/SUP], Wayne Paes[SUP] 4 [/SUP], Persephone Borrow[SUP] 4 [/SUP], Benedikt M Kessler[SUP] 2 4 [/SUP], Jeremy W Fry[SUP] 16 [/SUP], Nikolai F Schwabe[SUP] 16 [/SUP], Malcolm G Semple[SUP] 12 17 [/SUP], J Kenneth Baillie[SUP] 18 [/SUP], Shona C Moore[SUP] 12 [/SUP], Peter J M Openshaw[SUP] 19 [/SUP], M Azim Ansari[SUP] 4 [/SUP], Susanna Dunachie[SUP] 4 5 [/SUP], Eleanor Barnes[SUP] 4 5 20 [/SUP], John Frater[SUP] 4 5 [/SUP], Georgina Kerr[SUP] 4 [/SUP], Philip Goulder[SUP] 4 5 [/SUP], Teresa Lockett[SUP] 5 [/SUP], Robert Levin[SUP] 21 [/SUP], Yonghong Zhang[SUP] 2 6 [/SUP], Ronghua Jing[SUP] 6 [/SUP], Ling-Pei Ho[SUP] 1 2 4 20 [/SUP], Oxford Immunology Network Covid-19 Response T cell Consortium; ISARIC4C Investigators; Richard J Cornall[SUP] 1 4 5 [/SUP], Christopher P Conlon[SUP] 2 4 5 [/SUP], Paul Klenerman[SUP] 4 5 20 [/SUP], Gavin R Screaton[SUP] 4 5 20 [/SUP], Juthathip Mongkolsapaya[SUP] 2 4 20 22 [/SUP], Andrew McMichael[SUP] 2 4 [/SUP], Julian C Knight[SUP] 2 3 4 5 [/SUP], Graham Ogg[SUP] 1 2 5 20 [/SUP], Tao Dong[SUP] 23 24 25 [/SUP]
Collaborators, Affiliations
Abstract
The development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines and therapeutics will depend on understanding viral immunity. We studied T cell memory in 42 patients following recovery from COVID-19 (28 with mild disease and 14 with severe disease) and 16 unexposed donors, using interferon-γ-based assays with peptides spanning SARS-CoV-2 except ORF1. The breadth and magnitude of T cell responses were significantly higher in severe as compared with mild cases. Total and spike-specific T cell responses correlated with spike-specific antibody responses. We identified 41 peptides containing CD4[SUP]+[/SUP] and/or CD8[SUP]+[/SUP] epitopes, including six immunodominant regions. Six optimized CD8[SUP]+[/SUP] epitopes were defined, with peptide-MHC pentamer-positive cells displaying the central and effector memory phenotype. In mild cases, higher proportions of SARS-CoV-2-specific CD8[SUP]+[/SUP] T cells were observed. The identification of T cell responses associated with milder disease will support an understanding of protective immunity and highlights the potential of including non-spike proteins within future COVID-19 vaccine design.
. 2020 Sep 4.
doi: 10.1038/s41590-020-0782-6. Online ahead of print.
Broad and strong memory CD4 [SUP]+[/SUP] and CD8 [SUP]+[/SUP] T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19
Yanchun Peng[SUP] 1 2 [/SUP], Alexander J Mentzer[SUP] 3 4 5 [/SUP], Guihai Liu[SUP] 2 4 6 [/SUP], Xuan Yao[SUP] 1 2 4 [/SUP], Zixi Yin[SUP] 1 2 [/SUP], Danning Dong[SUP] 2 4 7 [/SUP], Wanwisa Dejnirattisai[SUP] 4 [/SUP], Timothy Rostron[SUP] 8 [/SUP], Piyada Supasa[SUP] 4 [/SUP], Chang Liu[SUP] 2 4 [/SUP], C?sar L?pez-Camacho[SUP] 3 4 [/SUP], Jose Slon-Campos[SUP] 4 [/SUP], Yuguang Zhao[SUP] 4 [/SUP], David I Stuart[SUP] 2 3 4 9 [/SUP], Guido C Paesen[SUP] 3 [/SUP], Jonathan M Grimes[SUP] 3 4 9 [/SUP], Alfred A Antson[SUP] 10 [/SUP], Oliver W Bayfield[SUP] 10 [/SUP], Dorothy E D P Hawkins[SUP] 10 [/SUP], De-Sheng Ker[SUP] 10 [/SUP], Beibei Wang[SUP] 2 4 [/SUP], Lance Turtle[SUP] 11 12 [/SUP], Krishanthi Subramaniam[SUP] 12 [/SUP], Paul Thomson[SUP] 12 [/SUP], Ping Zhang[SUP] 4 [/SUP], Christina Dold[SUP] 13 14 [/SUP], Jeremy Ratcliff[SUP] 4 [/SUP], Peter Simmonds[SUP] 4 [/SUP], Thushan de Silva[SUP] 15 [/SUP], Paul Sopp[SUP] 8 [/SUP], Dannielle Wellington[SUP] 1 2 [/SUP], Ushani Rajapaksa[SUP] 2 4 [/SUP], Yi-Ling Chen[SUP] 1 [/SUP], Mariolina Salio[SUP] 1 [/SUP], Giorgio Napolitani[SUP] 1 [/SUP], Wayne Paes[SUP] 4 [/SUP], Persephone Borrow[SUP] 4 [/SUP], Benedikt M Kessler[SUP] 2 4 [/SUP], Jeremy W Fry[SUP] 16 [/SUP], Nikolai F Schwabe[SUP] 16 [/SUP], Malcolm G Semple[SUP] 12 17 [/SUP], J Kenneth Baillie[SUP] 18 [/SUP], Shona C Moore[SUP] 12 [/SUP], Peter J M Openshaw[SUP] 19 [/SUP], M Azim Ansari[SUP] 4 [/SUP], Susanna Dunachie[SUP] 4 5 [/SUP], Eleanor Barnes[SUP] 4 5 20 [/SUP], John Frater[SUP] 4 5 [/SUP], Georgina Kerr[SUP] 4 [/SUP], Philip Goulder[SUP] 4 5 [/SUP], Teresa Lockett[SUP] 5 [/SUP], Robert Levin[SUP] 21 [/SUP], Yonghong Zhang[SUP] 2 6 [/SUP], Ronghua Jing[SUP] 6 [/SUP], Ling-Pei Ho[SUP] 1 2 4 20 [/SUP], Oxford Immunology Network Covid-19 Response T cell Consortium; ISARIC4C Investigators; Richard J Cornall[SUP] 1 4 5 [/SUP], Christopher P Conlon[SUP] 2 4 5 [/SUP], Paul Klenerman[SUP] 4 5 20 [/SUP], Gavin R Screaton[SUP] 4 5 20 [/SUP], Juthathip Mongkolsapaya[SUP] 2 4 20 22 [/SUP], Andrew McMichael[SUP] 2 4 [/SUP], Julian C Knight[SUP] 2 3 4 5 [/SUP], Graham Ogg[SUP] 1 2 5 20 [/SUP], Tao Dong[SUP] 23 24 25 [/SUP]
Collaborators, Affiliations
- PMID: 32887977
- DOI: 10.1038/s41590-020-0782-6
Abstract
The development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines and therapeutics will depend on understanding viral immunity. We studied T cell memory in 42 patients following recovery from COVID-19 (28 with mild disease and 14 with severe disease) and 16 unexposed donors, using interferon-γ-based assays with peptides spanning SARS-CoV-2 except ORF1. The breadth and magnitude of T cell responses were significantly higher in severe as compared with mild cases. Total and spike-specific T cell responses correlated with spike-specific antibody responses. We identified 41 peptides containing CD4[SUP]+[/SUP] and/or CD8[SUP]+[/SUP] epitopes, including six immunodominant regions. Six optimized CD8[SUP]+[/SUP] epitopes were defined, with peptide-MHC pentamer-positive cells displaying the central and effector memory phenotype. In mild cases, higher proportions of SARS-CoV-2-specific CD8[SUP]+[/SUP] T cells were observed. The identification of T cell responses associated with milder disease will support an understanding of protective immunity and highlights the potential of including non-spike proteins within future COVID-19 vaccine design.