tetano
Editor, Senior Moderator
Nanomedicine: Nanotechnology, Biology and Medicine
Available online 27 August 2013
In Press, Accepted Manuscript ? Note to users
Cover image
Nanoclusters self-assembled from conformation-stabilized influenza M2e as broadly cross-protective influenza vaccines
Li Wanga,
Annie Hessb,
Timothy Z. Changb,
Ying-Chun Wanga,
Julie A. Championb, Corresponding author contact information, E-mail the corresponding author,
Richard W. Compansa, Corresponding author contact information, E-mail the corresponding author,
Bao-Zhong Wanga, Corresponding author contact information, E-mail the corresponding author
a Department of Microbiology and Immunology, and Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia
b Georgia Institute of Technology, School of Chemical & Biomolecular Engineering, Atlanta, Georgia
Abstract
Influenza vaccines with broad cross-protection are urgently needed. The highly conserved ectodomain of the influenza matrix protein 2 (M2e) can be a promising candidate if its low immunogenicity was overcome. In this study, we generated protein nanoclusters self-assembled from conformation-stabilized M2e tetramers (tM2e) to improve its immunogenicity. The resulting nanoclusters showed an average hydrodynamic diameter of 268 nm. Vaccination with the nanoclusters by an intranasal route elicited high levels of serum antigen-specific IgG in mice (approximately 100-fold higher than that obtained with soluble tM2e), as well as antigen-specific T cell and mucosal antibody responses. The immunity conferred complete protection against lethal challenge with homo- as well as heterosubtypic viruses. These results demonstrate that nanoclusters assembled from conformation-stabilized M2e are promising as a potential universal influenza A vaccine. Self-assembly into nanoclusters represents a novel approach for increasing the immunogenicity of vaccine antigens.
http://www.sciencedirect.com/science/article/pii/S1549963413003651
Available online 27 August 2013
In Press, Accepted Manuscript ? Note to users
Cover image
Nanoclusters self-assembled from conformation-stabilized influenza M2e as broadly cross-protective influenza vaccines
Li Wanga,
Annie Hessb,
Timothy Z. Changb,
Ying-Chun Wanga,
Julie A. Championb, Corresponding author contact information, E-mail the corresponding author,
Richard W. Compansa, Corresponding author contact information, E-mail the corresponding author,
Bao-Zhong Wanga, Corresponding author contact information, E-mail the corresponding author
a Department of Microbiology and Immunology, and Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia
b Georgia Institute of Technology, School of Chemical & Biomolecular Engineering, Atlanta, Georgia
Abstract
Influenza vaccines with broad cross-protection are urgently needed. The highly conserved ectodomain of the influenza matrix protein 2 (M2e) can be a promising candidate if its low immunogenicity was overcome. In this study, we generated protein nanoclusters self-assembled from conformation-stabilized M2e tetramers (tM2e) to improve its immunogenicity. The resulting nanoclusters showed an average hydrodynamic diameter of 268 nm. Vaccination with the nanoclusters by an intranasal route elicited high levels of serum antigen-specific IgG in mice (approximately 100-fold higher than that obtained with soluble tM2e), as well as antigen-specific T cell and mucosal antibody responses. The immunity conferred complete protection against lethal challenge with homo- as well as heterosubtypic viruses. These results demonstrate that nanoclusters assembled from conformation-stabilized M2e are promising as a potential universal influenza A vaccine. Self-assembly into nanoclusters represents a novel approach for increasing the immunogenicity of vaccine antigens.
http://www.sciencedirect.com/science/article/pii/S1549963413003651