tetano
Editor, Senior Moderator
Nano Today
. 2021 Mar 19;101139.
doi: 10.1016/j.nantod.2021.101139. Online ahead of print.
Engineering a Self-Navigated MnARK Nanovaccine for Inducing Potent Protective Immunity against Novel Coronavirus
Yaling Wang[SUP] 1 2 [/SUP], Yuping Xie[SUP] 3 [/SUP], Jia Luo[SUP] 3 [/SUP], Mengyu Guo[SUP] 1 [/SUP], Xuhao Hu[SUP] 1 4 [/SUP], Xi Chen[SUP] 1 5 [/SUP], Ziwei Chen[SUP] 1 4 [/SUP], Xinyi Lu[SUP] 1 4 [/SUP], Lichun Mao[SUP] 1 4 [/SUP], Kai Zhang[SUP] 6 [/SUP], Liangnian Wei[SUP] 1 7 [/SUP], Yunfei Ma[SUP] 3 [/SUP], Ruixin Wang[SUP] 3 [/SUP], Jia Zhou[SUP] 3 8 [/SUP], Chunyan He[SUP] 3 [/SUP], Yufang Zhang[SUP] 3 [/SUP], Ye Zhang[SUP] 3 [/SUP], Sisi Chen[SUP] 3 [/SUP], Lijuan Shen[SUP] 3 [/SUP], Yun Chen[SUP] 7 [/SUP], Nasha Qiu[SUP] 1 [/SUP], Ying Liu[SUP] 1 [/SUP], Yanyan Cui[SUP] 9 [/SUP], Guoyang Liao[SUP] 3 [/SUP], Ye Liu[SUP] 3 [/SUP], Chunying Chen[SUP] 1 4 2 10 [/SUP]
Affiliations
Abstract
Effective vaccines are vital to the fight against the COVID-19 global pandemic. As a critical component of a subunit vaccine, the adjuvant is responsible for strengthening the antigen-induced immune responses. Here, we present a new nanovaccine that comprising the Receptor-Binding Domain (RBD) of spike protein and the manganese nanoadjuvant (MnARK), which induces humoral and cellular responses. Notably, even at a 5-fold lower antigen dose and with fewer injections, mice immunized with the MnARK vaccine immunized mice showed stronger neutralizing abilities against the infection of the pseudovirus (~270-fold) and live coronavirus (>8-fold) in vitro than that of Alum-adsorbed RBD vaccine (Alu-RBD). Furthermore, we found that the effective co-delivery of RBD antigen and MnARK to lymph nodes (LNs) elicited an increased cellular internalization and the activation of immune cells, including DC cells, CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T lymphocytes. Our findings highlight the importance of MnARK adjuvant in the design of novel coronavirus vaccines and provide a rationale strategy to design protective vaccines through promoting cellular internalization and the activation of immune-related pathways.
. 2021 Mar 19;101139.
doi: 10.1016/j.nantod.2021.101139. Online ahead of print.
Engineering a Self-Navigated MnARK Nanovaccine for Inducing Potent Protective Immunity against Novel Coronavirus
Yaling Wang[SUP] 1 2 [/SUP], Yuping Xie[SUP] 3 [/SUP], Jia Luo[SUP] 3 [/SUP], Mengyu Guo[SUP] 1 [/SUP], Xuhao Hu[SUP] 1 4 [/SUP], Xi Chen[SUP] 1 5 [/SUP], Ziwei Chen[SUP] 1 4 [/SUP], Xinyi Lu[SUP] 1 4 [/SUP], Lichun Mao[SUP] 1 4 [/SUP], Kai Zhang[SUP] 6 [/SUP], Liangnian Wei[SUP] 1 7 [/SUP], Yunfei Ma[SUP] 3 [/SUP], Ruixin Wang[SUP] 3 [/SUP], Jia Zhou[SUP] 3 8 [/SUP], Chunyan He[SUP] 3 [/SUP], Yufang Zhang[SUP] 3 [/SUP], Ye Zhang[SUP] 3 [/SUP], Sisi Chen[SUP] 3 [/SUP], Lijuan Shen[SUP] 3 [/SUP], Yun Chen[SUP] 7 [/SUP], Nasha Qiu[SUP] 1 [/SUP], Ying Liu[SUP] 1 [/SUP], Yanyan Cui[SUP] 9 [/SUP], Guoyang Liao[SUP] 3 [/SUP], Ye Liu[SUP] 3 [/SUP], Chunying Chen[SUP] 1 4 2 10 [/SUP]
Affiliations
- PMID: 33758593
- PMCID: PMC7972805
- DOI: 10.1016/j.nantod.2021.101139
Abstract
Effective vaccines are vital to the fight against the COVID-19 global pandemic. As a critical component of a subunit vaccine, the adjuvant is responsible for strengthening the antigen-induced immune responses. Here, we present a new nanovaccine that comprising the Receptor-Binding Domain (RBD) of spike protein and the manganese nanoadjuvant (MnARK), which induces humoral and cellular responses. Notably, even at a 5-fold lower antigen dose and with fewer injections, mice immunized with the MnARK vaccine immunized mice showed stronger neutralizing abilities against the infection of the pseudovirus (~270-fold) and live coronavirus (>8-fold) in vitro than that of Alum-adsorbed RBD vaccine (Alu-RBD). Furthermore, we found that the effective co-delivery of RBD antigen and MnARK to lymph nodes (LNs) elicited an increased cellular internalization and the activation of immune cells, including DC cells, CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T lymphocytes. Our findings highlight the importance of MnARK adjuvant in the design of novel coronavirus vaccines and provide a rationale strategy to design protective vaccines through promoting cellular internalization and the activation of immune-related pathways.