Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs

[tetano]

Obtaining suitable seed viruses for influenza vaccines poses a challenge for public health authorities and manufacturers. We used reverse genetics to generate vaccine seed-compatible viruses from the 2009 pandemic swine-origin influenza virus.

Comparison of viruses recovered with variations in residues 186 and 194 (based on the H3 numbering system) of the viral hemagglutinin showed that these viruses differed with respect to their ability to grow in eggs and cultured cells. Thus, we have demonstrated that molecular cloning of members of a quasispecies can help in selection of seed viruses for vaccine manufacture.

Author: Pirada SuphaphiphatMichael FrantiArmin HekeleAnders LiljaTerika SpencerEthan SettembreGene PalmerStefania CrottaAnnunziata TuccinoBjoern KeinerHeidi TrusheimKara BalabanisMelissa SackalMithra RothfederChristian MandlPhilip DormitzerPeter Mason




Published on: 2010-07-14

http://7thspace.com/headlines/35069...ove_replication_in_cell_culture_and_eggs.html

 

[gsgs]

Re: Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs

http://www.virologyj.com/content/pdf/1743-422x-7-157.pdf
P186,L194
S186,I194
S186,L194 (GQ117044)

+14 for H1-numbering

TCT=S
CTC=L

CCT=P - T598C Swine,Illinois abd the (original) Sw/4/Mexico have it
ATC=I - C622A in Singapore