tetano
Editor, Senior Moderator
Mucosal Immunol
. 2022 Feb 10.
doi: 10.1038/s41385-021-00478-4. Online ahead of print.
Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells
Veronica Martini[SUP] 1 2 3 [/SUP], Matthew Edmans[SUP] 4 [/SUP], Simon Gubbins[SUP] 4 [/SUP], Siddharth Jayaraman[SUP] 5 [/SUP], Basudev Paudyal[SUP] 4 [/SUP], Sophie Morgan[SUP] 4 [/SUP], Adam McNee[SUP] 4 [/SUP], Théo Morin[SUP] 6 [/SUP], Pramila Rijal[SUP] 7 [/SUP], Wilhelm Gerner[SUP] 4 [/SUP], Andrew K Sewell[SUP] 6 [/SUP], Ryo Inoue[SUP] 8 [/SUP], Mick Bailey[SUP] 9 [/SUP], Timothy Connelley[SUP] 5 [/SUP], Bryan Charleston[SUP] 4 [/SUP], Alain Townsend[SUP] 7 [/SUP], Peter Beverley[SUP] 10 [/SUP], Elma Tchilian[SUP] 11 [/SUP]
Affiliations
Abstract
For the first time we have defined naïve, central memory, effector memory and differentiated effector porcine CD8 T cells and analyzed their distribution in lymphoid and respiratory tissues after influenza infection or immunization, using peptide-MHC tetramers of three influenza nucleoprotein (NP) epitopes. The hierarchy of response to the three epitopes changes during the response in different tissues. Most NP-specific CD8 T cells in broncho-alveolar lavage (BAL) and lung are tissue resident memory cells (TRM) that express CD69 and downregulate CD45RA and CCR7. NP-specific cells isolated from BAL express genes characteristic of TRM, but gene expression differs at 7, 21 and 63 days post infection. In all tissues the frequency of NP-specific CD8 cells declines over 63 days almost to background levels but is best maintained in BAL. The kinetic of influenza specific memory CD8 T cell in this natural host species differs from that in small animal models.
. 2022 Feb 10.
doi: 10.1038/s41385-021-00478-4. Online ahead of print.
Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells
Veronica Martini[SUP] 1 2 3 [/SUP], Matthew Edmans[SUP] 4 [/SUP], Simon Gubbins[SUP] 4 [/SUP], Siddharth Jayaraman[SUP] 5 [/SUP], Basudev Paudyal[SUP] 4 [/SUP], Sophie Morgan[SUP] 4 [/SUP], Adam McNee[SUP] 4 [/SUP], Théo Morin[SUP] 6 [/SUP], Pramila Rijal[SUP] 7 [/SUP], Wilhelm Gerner[SUP] 4 [/SUP], Andrew K Sewell[SUP] 6 [/SUP], Ryo Inoue[SUP] 8 [/SUP], Mick Bailey[SUP] 9 [/SUP], Timothy Connelley[SUP] 5 [/SUP], Bryan Charleston[SUP] 4 [/SUP], Alain Townsend[SUP] 7 [/SUP], Peter Beverley[SUP] 10 [/SUP], Elma Tchilian[SUP] 11 [/SUP]
Affiliations
- PMID: 35145208
- DOI: 10.1038/s41385-021-00478-4
Abstract
For the first time we have defined naïve, central memory, effector memory and differentiated effector porcine CD8 T cells and analyzed their distribution in lymphoid and respiratory tissues after influenza infection or immunization, using peptide-MHC tetramers of three influenza nucleoprotein (NP) epitopes. The hierarchy of response to the three epitopes changes during the response in different tissues. Most NP-specific CD8 T cells in broncho-alveolar lavage (BAL) and lung are tissue resident memory cells (TRM) that express CD69 and downregulate CD45RA and CCR7. NP-specific cells isolated from BAL express genes characteristic of TRM, but gene expression differs at 7, 21 and 63 days post infection. In all tissues the frequency of NP-specific CD8 cells declines over 63 days almost to background levels but is best maintained in BAL. The kinetic of influenza specific memory CD8 T cell in this natural host species differs from that in small animal models.