tetano
Editor, Senior Moderator
J Vet Sci. 2015 Aug 4. [Epub ahead of print]
[h=1]Molecular characterization of highly pathogenic avian influenza H5N8 viruses isolated from Baikal teals found dead during a 2014 outbreak in Korea.[/h] Kim SH[SUP]1[/SUP], Hur M[SUP]2[/SUP], Suh JH[SUP]1[/SUP], Woo C[SUP]1[/SUP], Wang SJ[SUP]1[/SUP], Park ER[SUP]1[/SUP], Hwang J[SUP]1[/SUP], An IJ[SUP]1[/SUP], Jo SD[SUP]1[/SUP], Shin JH[SUP]1[/SUP], Yu SD[SUP]1[/SUP], Choi K[SUP]1[/SUP], Lee DH[SUP]3[/SUP], Song CS[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Nineteen highly pathogenic avian influenza (HPAI) H5N8 viruses were isolated from wild birds in the Donglim reservoir in Gochang, Jeonbuk province in Korea, which was first reported to be an outbreak site on January 17, 2014. Most genes from the nineteen viruses shared high nucleotide sequence identities, i.e., from 99.7% to 100%. Phylogenetic analysis showed that these viruses were reassortants of the HPAI H5 subtype and the H4N2 strain and that their hemagglutinin clade was 2.3.4.4, which originated from Eastern China. The hemagglutinin protein contained Q222 and G224 at the receptor-binding site. Although the neuraminidase protein contained I314V and the matrix 2 protein contained an S31N substitution, other mutations resulting in oseltamivir and amantadine resistance were not detected. No substitutions associated with increased virulence and enhanced transmission in mammals were detected in the polymerase basic protein 2 (627E and 701D). The non- structural-1 was 237 amino acids long and had the ESEV motif with additional RGNKMAD amino acids in the C terminal region. These viruses caused deaths in the baikal teal, which was unusual, and outbreaks occurred at the same time in both poultry and wild birds. These data are helpful for epidemiological understanding of HPAI and the design of prevention strategies.
[h=4]KEYWORDS:[/h] H5N8; avian influenza; phylogenetic analysis; surveillance; wild bird
PMID: 26245355 [PubMed - as supplied by publisher]
[h=1]Molecular characterization of highly pathogenic avian influenza H5N8 viruses isolated from Baikal teals found dead during a 2014 outbreak in Korea.[/h] Kim SH[SUP]1[/SUP], Hur M[SUP]2[/SUP], Suh JH[SUP]1[/SUP], Woo C[SUP]1[/SUP], Wang SJ[SUP]1[/SUP], Park ER[SUP]1[/SUP], Hwang J[SUP]1[/SUP], An IJ[SUP]1[/SUP], Jo SD[SUP]1[/SUP], Shin JH[SUP]1[/SUP], Yu SD[SUP]1[/SUP], Choi K[SUP]1[/SUP], Lee DH[SUP]3[/SUP], Song CS[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Nineteen highly pathogenic avian influenza (HPAI) H5N8 viruses were isolated from wild birds in the Donglim reservoir in Gochang, Jeonbuk province in Korea, which was first reported to be an outbreak site on January 17, 2014. Most genes from the nineteen viruses shared high nucleotide sequence identities, i.e., from 99.7% to 100%. Phylogenetic analysis showed that these viruses were reassortants of the HPAI H5 subtype and the H4N2 strain and that their hemagglutinin clade was 2.3.4.4, which originated from Eastern China. The hemagglutinin protein contained Q222 and G224 at the receptor-binding site. Although the neuraminidase protein contained I314V and the matrix 2 protein contained an S31N substitution, other mutations resulting in oseltamivir and amantadine resistance were not detected. No substitutions associated with increased virulence and enhanced transmission in mammals were detected in the polymerase basic protein 2 (627E and 701D). The non- structural-1 was 237 amino acids long and had the ESEV motif with additional RGNKMAD amino acids in the C terminal region. These viruses caused deaths in the baikal teal, which was unusual, and outbreaks occurred at the same time in both poultry and wild birds. These data are helpful for epidemiological understanding of HPAI and the design of prevention strategies.
[h=4]KEYWORDS:[/h] H5N8; avian influenza; phylogenetic analysis; surveillance; wild bird
PMID: 26245355 [PubMed - as supplied by publisher]