tetano
Editor, Senior Moderator
J Med Virol. 2017 Oct 26. doi: 10.1002/jmv.24982. [Epub ahead of print]
[h=1]Molecular and Epidemiological analysis of Pandemic and Post-pandemic Influenza A(H1N1)pdm09 virus from central India.[/h] Sahu M[SUP]1[/SUP], Singh N[SUP]1[/SUP], Shukla MK[SUP]1[/SUP], Potdar VA[SUP]2[/SUP], Sharma RK[SUP]1[/SUP], Sahare LK[SUP]1[/SUP], Ukey MJ[SUP]1[/SUP], Barde PV[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A(H1N1)pdm09 virus pandemic struck India in 2009 and continues to cause outbreaks in its post pandemic phase. Diminutive information is available about influenza A(H1N1)pdm09 from central India. This observational study presents epidemiological and molecular findings for the period of six years. Throat swab samples referred from districts of Madhya Pradesh were subjected to diagnosis of Influenza A(H1N1)pdm09 following WHO guidelines. Clinical and epidemiological data was recorded and analysed. Hemagglutinin (HA) gene sequencing and phylogenetic analysis was performed. The H275Y mutation responsible for antiviral resistance was tested using allelic real time RT-PCR. Out of 7365 tested samples 2406 (32.7%) were positive for Influenza A(H1N1)pdm09 of which 363 (15.08%) succumbed to infection. Significant trends was observed in positivity (x[SUP]2[/SUP] = 50.8; p<0.001) and mortality (x[SUP]2[/SUP] = 24.4; p<0.001) with increasing age. Mutations having clinical and epidemiological importance were detected. Phylogenetic analysis of HA gene sequences revealed that clade 7, 6 A and 6B viruses were in circulation. Oseltamivir resistance was detected in three fatal cases. Influenza A(H1N1)pdm09 viruses having genetic diversity were detected from central India and continues to be a concern for public health. Study highlights the need of year-round monitoring by establishment of strong molecular and clinical surveillance program. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Central India; Epidemiology; Influenza A(H1N1)pdm09; Mutations; Phylogenetic analysis
PMID: 29073730 DOI: 10.1002/jmv.24982
[h=1]Molecular and Epidemiological analysis of Pandemic and Post-pandemic Influenza A(H1N1)pdm09 virus from central India.[/h] Sahu M[SUP]1[/SUP], Singh N[SUP]1[/SUP], Shukla MK[SUP]1[/SUP], Potdar VA[SUP]2[/SUP], Sharma RK[SUP]1[/SUP], Sahare LK[SUP]1[/SUP], Ukey MJ[SUP]1[/SUP], Barde PV[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A(H1N1)pdm09 virus pandemic struck India in 2009 and continues to cause outbreaks in its post pandemic phase. Diminutive information is available about influenza A(H1N1)pdm09 from central India. This observational study presents epidemiological and molecular findings for the period of six years. Throat swab samples referred from districts of Madhya Pradesh were subjected to diagnosis of Influenza A(H1N1)pdm09 following WHO guidelines. Clinical and epidemiological data was recorded and analysed. Hemagglutinin (HA) gene sequencing and phylogenetic analysis was performed. The H275Y mutation responsible for antiviral resistance was tested using allelic real time RT-PCR. Out of 7365 tested samples 2406 (32.7%) were positive for Influenza A(H1N1)pdm09 of which 363 (15.08%) succumbed to infection. Significant trends was observed in positivity (x[SUP]2[/SUP] = 50.8; p<0.001) and mortality (x[SUP]2[/SUP] = 24.4; p<0.001) with increasing age. Mutations having clinical and epidemiological importance were detected. Phylogenetic analysis of HA gene sequences revealed that clade 7, 6 A and 6B viruses were in circulation. Oseltamivir resistance was detected in three fatal cases. Influenza A(H1N1)pdm09 viruses having genetic diversity were detected from central India and continues to be a concern for public health. Study highlights the need of year-round monitoring by establishment of strong molecular and clinical surveillance program. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Central India; Epidemiology; Influenza A(H1N1)pdm09; Mutations; Phylogenetic analysis
PMID: 29073730 DOI: 10.1002/jmv.24982