tetano
Editor, Senior Moderator
Mol Cells
. 2020 Nov 15.
doi: 10.14348/molcells.2020.0177. Online ahead of print.
Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2
Woo Ryung Kim[SUP] 1 2 3 [/SUP], Eun Gyung Park[SUP] 1 2 3 [/SUP], Kyung-Won Kang[SUP] 4 [/SUP], Sang-Myeong Lee[SUP] 4 5 [/SUP], Bumseok Kim[SUP] 6 [/SUP], Heui-Soo Kim[SUP] 2 7 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5?C, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. Five miRNAs (hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-16-5p, and hsa-miR-196a-1-3p) were found to commonly bind to SARS-CoV, MERS-CoV, and SARS-CoV-2. We also identified miRNAs that bind to receptor proteins, such as ACE2, ADAM17, and TMPRSS2, which are important for understanding the infection mechanism of SARS-CoV-2. The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. Especially, hsa-miR-15b-5p and hsa-miR-195-5p showed a large difference in expression, indicating that they may potentially be diagnostic biomarkers for SARS-CoV-2 infection.
Keywords: hamster lung; hsa-miR-15b-5p; hsa-miR-195-5p; microRNA; severe acute respiratory syndrome coronavirus 2.
. 2020 Nov 15.
doi: 10.14348/molcells.2020.0177. Online ahead of print.
Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2
Woo Ryung Kim[SUP] 1 2 3 [/SUP], Eun Gyung Park[SUP] 1 2 3 [/SUP], Kyung-Won Kang[SUP] 4 [/SUP], Sang-Myeong Lee[SUP] 4 5 [/SUP], Bumseok Kim[SUP] 6 [/SUP], Heui-Soo Kim[SUP] 2 7 [/SUP]
Affiliations
- PMID: 33199671
- DOI: 10.14348/molcells.2020.0177
Abstract
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5?C, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. Five miRNAs (hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-16-5p, and hsa-miR-196a-1-3p) were found to commonly bind to SARS-CoV, MERS-CoV, and SARS-CoV-2. We also identified miRNAs that bind to receptor proteins, such as ACE2, ADAM17, and TMPRSS2, which are important for understanding the infection mechanism of SARS-CoV-2. The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. Especially, hsa-miR-15b-5p and hsa-miR-195-5p showed a large difference in expression, indicating that they may potentially be diagnostic biomarkers for SARS-CoV-2 infection.
Keywords: hamster lung; hsa-miR-15b-5p; hsa-miR-195-5p; microRNA; severe acute respiratory syndrome coronavirus 2.