Modified Pulmonary Surfactant Is a Potent Adjuvant That Stimulates the Mucosal IgA Pr

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[SIZE=-1] http://www.jimmunol.org/cgi/content/abstract/176/2/1122

The Journal of Immunology, 2006, 176: 1122-1130.
Copyright ? 2006 by The American Association of Immunologists [/SIZE]
Modified Pulmonary Surfactant Is a Potent Adjuvant That Stimulates the Mucosal IgA Production in Response to the Influenza Virus Antigen¹

<nobr>Dai Mizuno</nobr>, <nobr>Mikiko Ide-Kurihara</nobr>, <nobr>Tomoko Ichinomiya</nobr>, <nobr>Itsuka Kubo</nobr> and <nobr>Hiroshi Kido²</nobr> [SIZE=-1] Division of Enzyme Chemistry, Institute for Enzyme Research, University of Tokushima, Tokushima, Japan [/SIZE]
<!-- ABS --> The intranasal administration of influenza hemagglutinin (HA)vaccine with Surfacten, a modified pulmonary surfactant freeof antigenic c-type lectins, as a mucosal adjuvant induced thehighest protective mucosal immunity in the airway. The intranasalimmunization of mice with HA vaccine (0.2 ?g)-Surfacten(0.2 ?g) selectively induced the neutralizing anti-HAIgA, but not IgG, and conferred nearly maximal protection inthe airway, without inducing a systemic response. In contrast,intranasal inoculation of vaccine with 0.2 ?g of the potentmucosal adjuvant cholera toxin B* (CT-B*), prepared by adding0.2% native CT to the B subunit of CT, induced both anti-HAIgA and IgG in the airway and in the serum. The intranasal administrationof HA vaccine alone induced a limited amount of mucosal IgAagainst influenza virus. Although the s.c. administration ofHA vaccine prominently induced serum IgG and IgA, Surfactenand CT-B* did not enhance their induction, and the concentrationsof Abs leaking into the airways were insufficient to preventviral multiplication. The intranasal administration of HA-Surfactenstimulated the expression of MHC class II, CD40, and CD86 moleculesin the CD11c-positive cells isolated from the nasal mucosa,but not the expression of cells from the lungs or spleens. Lymphocytesisolated from the airway mucosa after intranasal HA-Surfactenimmunization prominently induced TGF-

1 which, compared withinoculation without Surfacten, promoted an Ag-specific mucosalIgA response. Surfacten alone, however, did not induce TGF-

1.Our observations suggest that Surfacten, by mimicking the naturalsurfactant, is an effective mucosal adjuvant in the processof airway immunization.