tetano
Editor, Senior Moderator
Microsc Microanal
. 2022 Sep 6;1-25.
doi: 10.1017/S1431927622012430. Online ahead of print.
Ultracellular Imaging of Bronchoalveolar Lavage from Young COVID-19 Patients with Comorbidities Showed Greater SARS-COV-2 Infection but Lesser Ultrastructural Damage Than the Older Patients
Shikha Chaudhary[SUP] 1 [/SUP], Preeti Rai[SUP] 1 [/SUP], Arti Joshi[SUP] 1 [/SUP], Pooja Yadav[SUP] 2 [/SUP], Kishore Sesham[SUP] 1 [/SUP], Shailendra Kumar[SUP] 3 [/SUP], Asit Ranjan Mridha[SUP] 2 [/SUP], Upendra Baitha[SUP] 4 [/SUP], Tapas Chandra Nag[SUP] 1 [/SUP], Kapil Dev Soni[SUP] 5 [/SUP], Anjan Trikha[SUP] 3 [/SUP], Subhash Chandra Yadav[SUP] 1 [/SUP]
Affiliations
Abstract
In this study, we examined the cellular infectivity and ultrastructural changes due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in the various cells of bronchoalveolar fluid (BALF) from intubated patients of different age groups (≥60 years and <60 years) and with common comorbidities such as diabetes, liver and kidney diseases, and malignancies. BALF of 79 patients (38 cases >60 and 41 cases <60 years) were studied by light microscopy, immunofluorescence, scanning, and transmission electron microscopy to evaluate the ultrastructural changes in the ciliated epithelium, type II pneumocytes, macrophages, neutrophils, eosinophils, lymphocytes, and anucleated granulocytes. This study demonstrated relatively a greater infection and better preservation of subcellular structures in these cells from BALF of younger patients (<60 years compared with the older patients (≥60 years). The different cells of BALF from the patients without comorbidities showed higher viral load compared with the patients with comorbidities. Diabetic patients showed maximum ultrastructural damage in BALF cells in the comorbid group. This study highlights the comparative effect of SARS-CoV-2 infection on the different airway and inflammatory cells of BALF at the subcellular levels among older and younger patients and in patients with comorbid conditions.
Keywords: SARS-CoV-2; acute respiratory distress syndrome; electron microscopy; macrophage; neutrophil.
. 2022 Sep 6;1-25.
doi: 10.1017/S1431927622012430. Online ahead of print.
Ultracellular Imaging of Bronchoalveolar Lavage from Young COVID-19 Patients with Comorbidities Showed Greater SARS-COV-2 Infection but Lesser Ultrastructural Damage Than the Older Patients
Shikha Chaudhary[SUP] 1 [/SUP], Preeti Rai[SUP] 1 [/SUP], Arti Joshi[SUP] 1 [/SUP], Pooja Yadav[SUP] 2 [/SUP], Kishore Sesham[SUP] 1 [/SUP], Shailendra Kumar[SUP] 3 [/SUP], Asit Ranjan Mridha[SUP] 2 [/SUP], Upendra Baitha[SUP] 4 [/SUP], Tapas Chandra Nag[SUP] 1 [/SUP], Kapil Dev Soni[SUP] 5 [/SUP], Anjan Trikha[SUP] 3 [/SUP], Subhash Chandra Yadav[SUP] 1 [/SUP]
Affiliations
- PMID: 36065953
- DOI: 10.1017/S1431927622012430
Abstract
In this study, we examined the cellular infectivity and ultrastructural changes due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in the various cells of bronchoalveolar fluid (BALF) from intubated patients of different age groups (≥60 years and <60 years) and with common comorbidities such as diabetes, liver and kidney diseases, and malignancies. BALF of 79 patients (38 cases >60 and 41 cases <60 years) were studied by light microscopy, immunofluorescence, scanning, and transmission electron microscopy to evaluate the ultrastructural changes in the ciliated epithelium, type II pneumocytes, macrophages, neutrophils, eosinophils, lymphocytes, and anucleated granulocytes. This study demonstrated relatively a greater infection and better preservation of subcellular structures in these cells from BALF of younger patients (<60 years compared with the older patients (≥60 years). The different cells of BALF from the patients without comorbidities showed higher viral load compared with the patients with comorbidities. Diabetic patients showed maximum ultrastructural damage in BALF cells in the comorbid group. This study highlights the comparative effect of SARS-CoV-2 infection on the different airway and inflammatory cells of BALF at the subcellular levels among older and younger patients and in patients with comorbid conditions.
Keywords: SARS-CoV-2; acute respiratory distress syndrome; electron microscopy; macrophage; neutrophil.