tetano
Editor, Senior Moderator
Microbiome
. 2022 Apr 22;10(1):65.
doi: 10.1186/s40168-022-01260-9.
Human endogenous retrovirus K in the respiratory tract is associated with COVID-19 physiopathology
Jairo R Temerozo[SUP] #[/SUP][SUP] 1 2 3 4 [/SUP], Natalia Fintelman-Rodrigues[SUP] #[/SUP][SUP] 3 4 [/SUP], Monique Cristina Dos Santos[SUP] 3 [/SUP], Eugenio D Hottz[SUP] 3 5 [/SUP], Carolina Q Sacramento[SUP] 3 4 [/SUP], Aline de Paula Dias da Silva[SUP] 3 4 [/SUP], Samuel Coelho Mandacaru[SUP] 4 6 [/SUP], Emilly Caroline Dos Santos Moraes[SUP] 3 6 [/SUP], Monique R O Trugilho[SUP] 4 6 [/SUP], João S M Gesto[SUP] 4 [/SUP], Marcelo Alves Ferreira[SUP] 4 [/SUP], Felipe Betoni Saraiva[SUP] 7 [/SUP], Lohanna Palhinha[SUP] 3 [/SUP], Remy Martins-Gonçalves[SUP] 3 [/SUP], Isaclaudia Gomes Azevedo-Quintanilha[SUP] 3 [/SUP], Juliana L Abrantes[SUP] 8 [/SUP], Cássia Righy[SUP] 9 10 [/SUP], Pedro Kurtz[SUP] 9 11 [/SUP], Hui Jiang[SUP] 12 [/SUP], Hongdong Tan[SUP] 12 [/SUP], Carlos Morel[SUP] 4 [/SUP], Dumith Chequer Bou-Habib[SUP] 1 2 [/SUP], Fernando A Bozza[SUP] 10 11 [/SUP], Patrícia T Bozza[SUP] 3 [/SUP], Thiago Moreno L Souza[SUP] 13 14 [/SUP]
Affiliations
Abstract
Background: Critically ill 2019 coronavirus disease (COVID-19) patients under invasive mechanical ventilation (IMV) are 10 to 40 times more likely to die than the general population. Although progression from mild to severe COVID-19 has been associated with hypoxia, uncontrolled inflammation, and coagulopathy, the mechanisms involved in the progression to severity are poorly understood.
Methods: The virome of tracheal aspirates (TA) from 25 COVID-19 patients under IMV was assessed through unbiased RNA sequencing (RNA-seq), and correlation analyses were conducted using available clinical data. Unbiased sequences from nasopharyngeal swabs (NS) from mild cases and TA from non-COVID patients were included in our study for further comparisons.
Results: We found higher levels and differential expression of human endogenous retrovirus K (HERV-K) genes in TA from critically ill and deceased patients when comparing nasopharyngeal swabs from mild cases to TA from non-COVID patients. In critically ill patients, higher HERV-K levels were associated with early mortality (within 14 days of diagnosis) in the intensive care unit. Increased HERV-K expression in deceased patients was associated with IL-17-related inflammation, monocyte activation, and an increased consumption of clotting/fibrinolysis factors. Moreover, increased HERV-K expression was detected in human primary monocytes from healthy donors after experimental SARS-CoV-2 infection in vitro.
Conclusion: Our data implicate the levels of HERV-K transcripts in the physiopathology of COVID-19 in the respiratory tract of patients under invasive mechanical ventilation. Video abstract.
. 2022 Apr 22;10(1):65.
doi: 10.1186/s40168-022-01260-9.
Human endogenous retrovirus K in the respiratory tract is associated with COVID-19 physiopathology
Jairo R Temerozo[SUP] #[/SUP][SUP] 1 2 3 4 [/SUP], Natalia Fintelman-Rodrigues[SUP] #[/SUP][SUP] 3 4 [/SUP], Monique Cristina Dos Santos[SUP] 3 [/SUP], Eugenio D Hottz[SUP] 3 5 [/SUP], Carolina Q Sacramento[SUP] 3 4 [/SUP], Aline de Paula Dias da Silva[SUP] 3 4 [/SUP], Samuel Coelho Mandacaru[SUP] 4 6 [/SUP], Emilly Caroline Dos Santos Moraes[SUP] 3 6 [/SUP], Monique R O Trugilho[SUP] 4 6 [/SUP], João S M Gesto[SUP] 4 [/SUP], Marcelo Alves Ferreira[SUP] 4 [/SUP], Felipe Betoni Saraiva[SUP] 7 [/SUP], Lohanna Palhinha[SUP] 3 [/SUP], Remy Martins-Gonçalves[SUP] 3 [/SUP], Isaclaudia Gomes Azevedo-Quintanilha[SUP] 3 [/SUP], Juliana L Abrantes[SUP] 8 [/SUP], Cássia Righy[SUP] 9 10 [/SUP], Pedro Kurtz[SUP] 9 11 [/SUP], Hui Jiang[SUP] 12 [/SUP], Hongdong Tan[SUP] 12 [/SUP], Carlos Morel[SUP] 4 [/SUP], Dumith Chequer Bou-Habib[SUP] 1 2 [/SUP], Fernando A Bozza[SUP] 10 11 [/SUP], Patrícia T Bozza[SUP] 3 [/SUP], Thiago Moreno L Souza[SUP] 13 14 [/SUP]
Affiliations
- PMID: 35459226
- PMCID: PMC9024070
- DOI: 10.1186/s40168-022-01260-9
Abstract
Background: Critically ill 2019 coronavirus disease (COVID-19) patients under invasive mechanical ventilation (IMV) are 10 to 40 times more likely to die than the general population. Although progression from mild to severe COVID-19 has been associated with hypoxia, uncontrolled inflammation, and coagulopathy, the mechanisms involved in the progression to severity are poorly understood.
Methods: The virome of tracheal aspirates (TA) from 25 COVID-19 patients under IMV was assessed through unbiased RNA sequencing (RNA-seq), and correlation analyses were conducted using available clinical data. Unbiased sequences from nasopharyngeal swabs (NS) from mild cases and TA from non-COVID patients were included in our study for further comparisons.
Results: We found higher levels and differential expression of human endogenous retrovirus K (HERV-K) genes in TA from critically ill and deceased patients when comparing nasopharyngeal swabs from mild cases to TA from non-COVID patients. In critically ill patients, higher HERV-K levels were associated with early mortality (within 14 days of diagnosis) in the intensive care unit. Increased HERV-K expression in deceased patients was associated with IL-17-related inflammation, monocyte activation, and an increased consumption of clotting/fibrinolysis factors. Moreover, increased HERV-K expression was detected in human primary monocytes from healthy donors after experimental SARS-CoV-2 infection in vitro.
Conclusion: Our data implicate the levels of HERV-K transcripts in the physiopathology of COVID-19 in the respiratory tract of patients under invasive mechanical ventilation. Video abstract.