tetano
Editor, Senior Moderator
Microb Pathog
. 2023 Feb 1;176:106017.
doi: 10.1016/j.micpath.2023.106017. Online ahead of print.
Decreased expression of surfactant Protein-C and CD74 in alveolar epithelial cells during influenza virus A(H1N1)pdm09 and H3N2 infection
L I Ibañez[SUP] 1 [/SUP], V P Martinez[SUP] 2 [/SUP], A A Iglesias[SUP] 2 [/SUP], C M Bellomo[SUP] 2 [/SUP], D O Alonso[SUP] 2 [/SUP], R M Coelho[SUP] 2 [/SUP], L Martinez Peralta[SUP] 3 [/SUP], N Periolo[SUP] 4 [/SUP]
Affiliations
Abstract
The primary replication site of Influenza A virus (IAV) is type II alveolar epithelial cells (AECII), which are central to normal lung function and present important immune functions. Surfactant components are synthesized primarily by AECII, which play a crucial role in host defense against infection. The aim of this study was to analyze if the impact of influenza infection is differential between A(H1N1)pdm09 and A/Victoria/3/75 (H3N2) on costimulatory molecules and ProSP-C expression in AECII from BALB/c mice infected and A549 cell line infected with both strains. Pandemic A(H1N1)pdm09 and A/Victoria/3/75 (H3N2) were used to infect BALB/c mice and the A549 cell line. We evaluated the surface expression of co-stimulatory molecules (CD45/CD31/CD74/ProSP-C) in AECII and A549 cell lines. Our results showed a significant decrease in ProSP-C[SUP]+[/SUP] CD31[SUP]-[/SUP] CD45[SUP]-[/SUP] and CD74[SUP]+[/SUP] CD31[SUP]-[/SUP] CD45[SUP]-[/SUP] expression in AECII and A549 cell line with the virus strain A(H1N1)pdm09 versus A/Victoria/3/75 (H3N2) and controls (non-infection conditions). Our findings indicate that changes in the expression of ProSP-C in AECII and A549 cell lines in infection conditions could result in dysfunction leading to decreased lung compliance, increased work of breathing and increased susceptibility to injury.
Keywords: Alveolar epithelial type II cell; Influenza; Pulmonary surfactant.
. 2023 Feb 1;176:106017.
doi: 10.1016/j.micpath.2023.106017. Online ahead of print.
Decreased expression of surfactant Protein-C and CD74 in alveolar epithelial cells during influenza virus A(H1N1)pdm09 and H3N2 infection
L I Ibañez[SUP] 1 [/SUP], V P Martinez[SUP] 2 [/SUP], A A Iglesias[SUP] 2 [/SUP], C M Bellomo[SUP] 2 [/SUP], D O Alonso[SUP] 2 [/SUP], R M Coelho[SUP] 2 [/SUP], L Martinez Peralta[SUP] 3 [/SUP], N Periolo[SUP] 4 [/SUP]
Affiliations
- PMID: 36736545
- DOI: 10.1016/j.micpath.2023.106017
Abstract
The primary replication site of Influenza A virus (IAV) is type II alveolar epithelial cells (AECII), which are central to normal lung function and present important immune functions. Surfactant components are synthesized primarily by AECII, which play a crucial role in host defense against infection. The aim of this study was to analyze if the impact of influenza infection is differential between A(H1N1)pdm09 and A/Victoria/3/75 (H3N2) on costimulatory molecules and ProSP-C expression in AECII from BALB/c mice infected and A549 cell line infected with both strains. Pandemic A(H1N1)pdm09 and A/Victoria/3/75 (H3N2) were used to infect BALB/c mice and the A549 cell line. We evaluated the surface expression of co-stimulatory molecules (CD45/CD31/CD74/ProSP-C) in AECII and A549 cell lines. Our results showed a significant decrease in ProSP-C[SUP]+[/SUP] CD31[SUP]-[/SUP] CD45[SUP]-[/SUP] and CD74[SUP]+[/SUP] CD31[SUP]-[/SUP] CD45[SUP]-[/SUP] expression in AECII and A549 cell line with the virus strain A(H1N1)pdm09 versus A/Victoria/3/75 (H3N2) and controls (non-infection conditions). Our findings indicate that changes in the expression of ProSP-C in AECII and A549 cell lines in infection conditions could result in dysfunction leading to decreased lung compliance, increased work of breathing and increased susceptibility to injury.
Keywords: Alveolar epithelial type II cell; Influenza; Pulmonary surfactant.