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Med . Early-warning signals and the role of H9N2 in the spillover of avian influenza viruses

tetano

Editor, Senior Moderator
Med


. 2025 Mar 18:100639.
doi: 10.1016/j.medj.2025.100639. Online ahead of print. Early-warning signals and the role of H9N2 in the spillover of avian influenza viruses

Yan-He Wang[SUP] 1 [/SUP], Jin-Jin Chen[SUP] 2 [/SUP], Jun Ma[SUP] 3 [/SUP], Jonathan E Owen[SUP] 4 [/SUP], Guo-Lin Wang[SUP] 5 [/SUP], Lin-Jie Yu[SUP] 5 [/SUP], Chun-Xi Shan[SUP] 5 [/SUP], Yao Tian[SUP] 5 [/SUP], Chen-Long Lv[SUP] 5 [/SUP], Tao Wang[SUP] 6 [/SUP], Yan Zhang[SUP] 5 [/SUP], Sheng-Hong Lin[SUP] 5 [/SUP], Xin-Jing Zhao[SUP] 7 [/SUP], Sheng Zhang[SUP] 3 [/SUP], Wang-Qian Wei[SUP] 7 [/SUP], Yuan-Yuan Zhang[SUP] 8 [/SUP], Tian Tang[SUP] 5 [/SUP], Xin-Lou Li[SUP] 9 [/SUP], Tao Jiang[SUP] 5 [/SUP], Jing Li[SUP] 5 [/SUP], Xiao-Ai Zhang[SUP] 5 [/SUP], Feng Hong[SUP] 10 [/SUP], Simon I Hay[SUP] 11 [/SUP], Yan-Song Sun[SUP] 12 [/SUP], Wei Liu[SUP] 13 [/SUP], Li-Qun Fang[SUP] 14 [/SUP]



Affiliations
Abstract

Background: The spillover of avian influenza viruses (AIVs) presents a significant global public health threat, leading to unpredictable and recurring pandemics. Current pandemic assessment tools suffer from deficiencies in terms of timeliness, capability for automation, and ability to generate risk estimates for multiple subtypes in the absence of documented human cases.
Methods: To address these challenges, we created an integrated database encompassing global AIV-related data from 1981 to 2022. This database enabled us to estimate the rapid expansion of spatial range and host diversity for specific AIV subtypes, alongside their increasing prevalence in hosts that have close contact with humans. These factors were used as early-warning signals for potential AIV spillover. We analyzed spillover patterns of AIVs using machine learning models, spatial Durbin models, and phylogenetic analysis.
Findings: Our results indicate a high potential for future spillover by subtypes H3N1, H4N6, H5N2, H5N3, H6N2, and H11N9. Additionally, we identified a significant risk for re-emergence by subtypes H5N1, H5N6, H5N8, and H9N2. Furthermore, our analysis highlighted 12 key strains of H9N2 as internal genetic donors for human adaptation in AIVs, demonstrating the crucial role of H9N2 in facilitating AIV spillover.
Conclusions: These findings provide a foundation for rapidly identifying high-risk subtypes, thus optimizing resource allocation in vaccine manufacture. They also underscore the potential significance of reducing the prevalence of H9N2 as a complementary strategy to mitigate chances of AIV spillovers.
Funding: National Key Research and Development Program of China.

Keywords: H9N2; Translation to population health; avian influenza virus; emerging infectious diseases; geo-epidemiology; interspecies transmission; risk assessment; spillover; zoonosis.

 
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