tetano
Editor, Senior Moderator
Immunol Cell Biol. 2015 Aug 14. doi: 10.1038/icb.2015.81. [Epub ahead of print]
[h=1]Mapping the pulmonary environment of animals protected from virulent H1N1 influenza infection using the TLR-2 agonist Pam2Cys.[/h] Mifsud EJ[SUP]1[/SUP], Tan AC[SUP]1[/SUP], Reading PC[SUP]2[/SUP], Jackson DC[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We have previously shown that intranasal administration of the Toll-like receptor-2 (TLR-2) agonist, S-[2,3-bis(palmitoyloxy)propyl] cysteine (Pam[SUB]2[/SUB]Cys), provides immediate and antigen independent protection against challenge with influenza virus. Here we characterize the cellular pulmonary environments of mice which had either been treated with Pam[SUB]2[/SUB]Cys or placebo and then challenged with influenza virus. We show that Pam[SUB]2[/SUB]Cys treatment results in the influx of innate immune cells into the lungs and that depletion of phagocytic cells from this influx using clodronate-loaded liposomes caused a reduction in the number of interstitial macrophages and monocytes. This resulted in abolition of the protective effect indicating the importance of this cellular subset in Pam[SUB]2[/SUB]Cys-mediated protection.Immunology and Cell Biology accepted article preview online, 14 August 2015. doi:10.1038/icb.2015.81.
PMID: 26272554 [PubMed - as supplied by publisher]
[h=1]Mapping the pulmonary environment of animals protected from virulent H1N1 influenza infection using the TLR-2 agonist Pam2Cys.[/h] Mifsud EJ[SUP]1[/SUP], Tan AC[SUP]1[/SUP], Reading PC[SUP]2[/SUP], Jackson DC[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We have previously shown that intranasal administration of the Toll-like receptor-2 (TLR-2) agonist, S-[2,3-bis(palmitoyloxy)propyl] cysteine (Pam[SUB]2[/SUB]Cys), provides immediate and antigen independent protection against challenge with influenza virus. Here we characterize the cellular pulmonary environments of mice which had either been treated with Pam[SUB]2[/SUB]Cys or placebo and then challenged with influenza virus. We show that Pam[SUB]2[/SUB]Cys treatment results in the influx of innate immune cells into the lungs and that depletion of phagocytic cells from this influx using clodronate-loaded liposomes caused a reduction in the number of interstitial macrophages and monocytes. This resulted in abolition of the protective effect indicating the importance of this cellular subset in Pam[SUB]2[/SUB]Cys-mediated protection.Immunology and Cell Biology accepted article preview online, 14 August 2015. doi:10.1038/icb.2015.81.
PMID: 26272554 [PubMed - as supplied by publisher]