tetano
Editor, Senior Moderator
Influenza Other Respi Viruses. 2012 Jun 19. doi: 10.1111/j.1750-2659.2012.00392.x. [Epub ahead of print]
Many ways to make an influenza virus - review of influenza virus reverse genetics methods.
Engelhardt OG.
Source
Division of Virology, National Institute for Biological Standards and Control, Health Protection Agency, Potters Bar, UK.
Abstract
Please cite this paper as: Othmar G. Engelhardt (2012) Many ways to make an influenza virus - review of influenza virus reverse genetics methods. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750-2659.2012.00392.x. Methods to introduce targeted mutations into a genome or, in the context of virology, into a virus are subsumed under the term reverse genetics (RG). Influenza viruses are important human pathogens that continue to surprise us. The development of RG for influenza viruses has greatly expanded our knowledge about influenza virus and enabled researchers to generate influenza viruses with rationally designed genotypes. Currently, a wide array of influenza virus RG methods is available. These can all be traced to fundamental principles essential in any RG system for negative-strand RNA viruses. This review gives an overview of these principles and of the multitude of RG methods, categorising them by technical characteristics.
? 2012 Blackwell Publishing Ltd.
PMID:
22712782
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22712782
Many ways to make an influenza virus - review of influenza virus reverse genetics methods.
Engelhardt OG.
Source
Division of Virology, National Institute for Biological Standards and Control, Health Protection Agency, Potters Bar, UK.
Abstract
Please cite this paper as: Othmar G. Engelhardt (2012) Many ways to make an influenza virus - review of influenza virus reverse genetics methods. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750-2659.2012.00392.x. Methods to introduce targeted mutations into a genome or, in the context of virology, into a virus are subsumed under the term reverse genetics (RG). Influenza viruses are important human pathogens that continue to surprise us. The development of RG for influenza viruses has greatly expanded our knowledge about influenza virus and enabled researchers to generate influenza viruses with rationally designed genotypes. Currently, a wide array of influenza virus RG methods is available. These can all be traced to fundamental principles essential in any RG system for negative-strand RNA viruses. This review gives an overview of these principles and of the multitude of RG methods, categorising them by technical characteristics.
? 2012 Blackwell Publishing Ltd.
PMID:
22712782
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22712782