tetano
Editor, Senior Moderator
Respir Physiol Neurobiol. 2014 Jul 23. pii: S1569-9048(14)00184-0. doi: 10.1016/j.resp.2014.07.010. [Epub ahead of print]
Magnitude of influenza virus replication and cell damage is associated with interleukin-6 production in primary cultures of human tracheal epithelium.
Yamaya M1, Nadine LK2, Ota C2, Kubo H2, Makiguchi T3, Nagatomi R4, Nishimura H5.
Author information
Abstract
Primary cultures of human tracheal epithelium were infected with influenza viruses to examine the relationships between the magnitude of viral replication and infection-induced cell damage and cytokine production in airway epithelial cells. Infection with four strains of the type A influenza virus increased the detached cell number and lactate dehydrogenase (LDH) levels in the supernatants. The detached cell number and LDH levels were related to the viral titers and interleukin (IL)-6 levels and the nuclear factor kappa B (NF-κB) p65 activation. Treatment of the cells with an anti-IL-6 receptor antibody and an NF-κB inhibitor, caffeic acid phenethyl ester, reduced the detached cell number, viral titers and the LDH levels and improved cell viability after infection with the pandemic influenza virus [A/Sendai-H/N0633/2009 (H1N1) pdm09]. A caspase-3 inhibitor, benzyloxycarbonyl-DEVD-fluoromethyl ketone, reduced the detached cell number and viral titers. Influenza viral infection-induced cell damage may be partly related to the magnitude of viral replication, NF-κB-p65-mediated IL-6 production and caspase-3 activation.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Airway epithelial cells; Cell damage; Influenza virus; Interleukin-6; Pathogenicity
PMID:
25064661
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25064661
Magnitude of influenza virus replication and cell damage is associated with interleukin-6 production in primary cultures of human tracheal epithelium.
Yamaya M1, Nadine LK2, Ota C2, Kubo H2, Makiguchi T3, Nagatomi R4, Nishimura H5.
Author information
Abstract
Primary cultures of human tracheal epithelium were infected with influenza viruses to examine the relationships between the magnitude of viral replication and infection-induced cell damage and cytokine production in airway epithelial cells. Infection with four strains of the type A influenza virus increased the detached cell number and lactate dehydrogenase (LDH) levels in the supernatants. The detached cell number and LDH levels were related to the viral titers and interleukin (IL)-6 levels and the nuclear factor kappa B (NF-κB) p65 activation. Treatment of the cells with an anti-IL-6 receptor antibody and an NF-κB inhibitor, caffeic acid phenethyl ester, reduced the detached cell number, viral titers and the LDH levels and improved cell viability after infection with the pandemic influenza virus [A/Sendai-H/N0633/2009 (H1N1) pdm09]. A caspase-3 inhibitor, benzyloxycarbonyl-DEVD-fluoromethyl ketone, reduced the detached cell number and viral titers. Influenza viral infection-induced cell damage may be partly related to the magnitude of viral replication, NF-κB-p65-mediated IL-6 production and caspase-3 activation.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Airway epithelial cells; Cell damage; Influenza virus; Interleukin-6; Pathogenicity
PMID:
25064661
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25064661