tetano
Editor, Senior Moderator
Life Sci
. 2021 Jan 13;119046.
doi: 10.1016/j.lfs.2021.119046. Online ahead of print.
The proteomic characteristics of airway mucus from critical ill COVID-19 patients
Zili Zhang[SUP] 1 [/SUP], Tao Wang[SUP] 1 [/SUP], Fei Liu[SUP] 1 [/SUP], Airu Zhu[SUP] 1 [/SUP], Guoping Gu[SUP] 1 [/SUP], Jieping Luo[SUP] 1 [/SUP], Jingyi Xu[SUP] 1 [/SUP], Jincun Zhao[SUP] 1 [/SUP], Yiming Li[SUP] 1 [/SUP], Yuanyuan Li[SUP] 1 [/SUP], Xiaoqing Liu[SUP] 1 [/SUP], Nanshan Zhong[SUP] 1 [/SUP], Wenju Lu[SUP] 2 [/SUP]
Affiliations
Abstract
Background: The pandemic of the coronavirus disease 2019 (COVID-19) has brought a global public health crisis. However, the pathogenesis underlying COVID-19 are barely understood.
Methods: In this study, we performed proteomic analyses of airway mucus obtained by bronchoscopy from severe COVID-19 patients. In total, 2351 and 2073 proteins were identified and quantified in COVID-19 patients and healthy controls, respectively.
Results: Among them, 92 differentiated expressed proteins (DEPs) (46 up-regulated and 46 down-regulated) were found with a fold change >1.5 or <0.67 and a p-value <0.05, and 375 proteins were uniquely present in airway mucus from COVID-19 patients. Pathway and network enrichment analyses revealed that the 92 DEPs were mostly associated with metabolic, complement and coagulation cascades, lysosome, and cholesterol metabolism pathways, and the 375 COVID-19 only proteins were mainly enriched in amino acid degradation (Valine, Leucine and Isoleucine degradation), amino acid metabolism (beta-Alanine, Tryptophan, Cysteine and Methionine metabolism), oxidative phosphorylation, phagosome, and cholesterol metabolism pathways.
Conclusions: This study aims to provide fundamental data for elucidating proteomic changes of COVID-19, which may implicate further investigation of molecular targets directing at specific therapy.
Keywords: Airway mucus; COVID-19; Critical ill patients; Proteomic.
. 2021 Jan 13;119046.
doi: 10.1016/j.lfs.2021.119046. Online ahead of print.
The proteomic characteristics of airway mucus from critical ill COVID-19 patients
Zili Zhang[SUP] 1 [/SUP], Tao Wang[SUP] 1 [/SUP], Fei Liu[SUP] 1 [/SUP], Airu Zhu[SUP] 1 [/SUP], Guoping Gu[SUP] 1 [/SUP], Jieping Luo[SUP] 1 [/SUP], Jingyi Xu[SUP] 1 [/SUP], Jincun Zhao[SUP] 1 [/SUP], Yiming Li[SUP] 1 [/SUP], Yuanyuan Li[SUP] 1 [/SUP], Xiaoqing Liu[SUP] 1 [/SUP], Nanshan Zhong[SUP] 1 [/SUP], Wenju Lu[SUP] 2 [/SUP]
Affiliations
- PMID: 33453245
- DOI: 10.1016/j.lfs.2021.119046
Abstract
Background: The pandemic of the coronavirus disease 2019 (COVID-19) has brought a global public health crisis. However, the pathogenesis underlying COVID-19 are barely understood.
Methods: In this study, we performed proteomic analyses of airway mucus obtained by bronchoscopy from severe COVID-19 patients. In total, 2351 and 2073 proteins were identified and quantified in COVID-19 patients and healthy controls, respectively.
Results: Among them, 92 differentiated expressed proteins (DEPs) (46 up-regulated and 46 down-regulated) were found with a fold change >1.5 or <0.67 and a p-value <0.05, and 375 proteins were uniquely present in airway mucus from COVID-19 patients. Pathway and network enrichment analyses revealed that the 92 DEPs were mostly associated with metabolic, complement and coagulation cascades, lysosome, and cholesterol metabolism pathways, and the 375 COVID-19 only proteins were mainly enriched in amino acid degradation (Valine, Leucine and Isoleucine degradation), amino acid metabolism (beta-Alanine, Tryptophan, Cysteine and Methionine metabolism), oxidative phosphorylation, phagosome, and cholesterol metabolism pathways.
Conclusions: This study aims to provide fundamental data for elucidating proteomic changes of COVID-19, which may implicate further investigation of molecular targets directing at specific therapy.
Keywords: Airway mucus; COVID-19; Critical ill patients; Proteomic.